Melatonin prevents ethanol-induced gastric mucosal damage possibly due to its antioxidant effect
Melatonin prevents ethanol-induced gastric mucosal damage possibly due to its antioxidant effect
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DOI：
10.1023/a:1014764705864
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发表时间：
2002-04-01
影响因子：
3.1
通讯作者：
Bilici, S
中科院分区：
文献类型：
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作者：
Bilici, D;S端leyman, H;Bilici, S
氧自由基释放被认为是乙醇致急性胃损伤的一个致病因素。褪黑素是一种松果体激素，可以清除氧自由基。我们研究了肠外给药褪黑素是否能阻止乙醇诱导的大鼠胃粘膜宏观损伤、多形核（PMN）白细胞浸润、总谷胱甘肽（tGSH）浓度降低和谷胱甘肽还原酶（GSSG-Rd）活性。我们比较了褪黑素和奥美拉唑的效果。用三个不同的参数来评估乙醇诱导的粘膜损伤：胃总谷胱甘肽（tGSH）浓度和谷胱甘肽还原酶（GSSG-Rd）活性、PMN白细胞数量和宏观观察。乙醇处理后，胃tGSH浓度和GSSG-Rd活性降低，pmn数量增加。研究发现，褪黑素预处理可增加tGSH浓度和GSSG-Rd活性。褪黑素还能减少乙醇诱导的胃中PMN的浸润。乙醇对整个胃粘膜造成损伤。褪黑素显著降低乙醇引起的宏观损伤程度。总之，这些发现支持褪黑素对胃溃疡的保护可能是由于其抗氧化活性。
Oxygen radical release has been proposed as a pathogenic factor of the ethanol-induced acute gastric injury. Melatonin, a pineal hormone, is known to scavenge oxygen free radicals. We investigated whether parenteral administration of melatonin prevented ethanol-induced macroscopic damage, polymorphonuclear (PMN) leukocyte infiltration, depletion of total glutathione (tGSH) concentration, and glutathione reductase (GSSG-Rd) activity in the rat gastric mucosa. We compared the effects of melatonin with those of omeprazole. Ethanol-induced mucosal damage was evaluated using three different parameters: gastric total glutathione (tGSH) concentration and glutathione reductase (GSSG-Rd) activity, the number of PMN leukocytes, and macroscopic investigation. Gatric tGSH concentration and GSSG-Rd activity decreased and the number of PMNs increased after ethanol administration. It was found that pretreatment with melatonin increased both tGSH concentration and GSSG-Rd activity. Melatonin also reduced ethanol-induced PMN infiltration in the stomach. Ethanol administration damaged the entire gastric mucosa. Melatonin significantly decreased the extent of ethanol-induced macroscopic injury. In conclusion, these findings support the conclusion that the protection conferred by melatonin in gastric ulcer is presumably due to its antioxidant activity.