Biomarker Discovery in Low-Grade Breast Cancer Using Isobaric Stable Isotope Tags and Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry (iTRAQ-2DLC-MS/MS) Based Quantitative Proteomic Analysis
Biomarker Discovery in Low-Grade Breast Cancer Using Isobaric Stable Isotope Tags and Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry (iTRAQ-2DLC-MS/MS) Based Quantitative Proteomic Analysis
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DOI：
10.1021/pr800622b
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发表时间：
2009-01-01
影响因子：
4.4
通讯作者：
Garbis, Spiros D.
中科院分区：
文献类型：
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作者：
Bouchal, Pavel;Roumeliotis, Theodoros;Garbis, Spiros D.
本初步研究构成了使用基于定量LC-MS/MS的蛋白质组学方法比较分析代表性低级别乳腺原发性肿瘤组织（伴和不伴转移和淋巴结转移）与非转移性肿瘤类型的原理验证。研究方法采用iTRAQ稳定同位素标记、二维液相色谱、纳米电喷雾电离和使用混合QqTOF平台（iTRAQ-2DLC-MS/MS）的高分辨率串联质谱。本研究的主要目的是（1）定义使用这种方法可获得的蛋白质谱，以及（2）突出验证和确认研究的潜在候选者，这些研究集中在乳腺癌转移过程中涉及的生物标志物上。该研究导致了605个非冗余蛋白质的可重复鉴定（p
The present pilot study constitutes a proof-of-principle in the use of a quantitative LC-MS/MS based proteomic method for the comparative analysis of representative low-grade breast primary tumor tissues with and without metastases and metastasis in lymph node relative to the nonmetastatic tumor type. The study method incorporated iTRAQ stable isotope labeling, two-dimensional liquid chromatography, nanoelectrospray ionization and high resolution tandem mass spectrometry using the hybrid QqTOF platform (iTRAQ-2DLC-MS/MS). The principal aims of this study were (1) to define the protein spectrum obtainable using this approach, and (2) to highlight potential candidates for verification and validation studies focused on biomarkers involved in metastatic processes in breast cancer. The study resulted in the reproducible identification of 605 nonredundant proteins (p