Assembly and immunogenicity of human papillomavirus type 16 major capsid protein(HPV16 L1) in Pichia pastoris
Assembly and immunogenicity of human papillomavirus type 16 major capsid protein(HPV16 L1) in Pichia pastoris
复制标题
人乳头瘤病毒16型主要衣壳蛋白(HPV16 L1)在毕赤酵母中的组装及免疫原性
DOI：
10.1016/s1005-9040(07)60042-7
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发表时间：
2007-03-01
影响因子：
3.1
通讯作者：
Kong Wei
中科院分区：
文献类型：
--
作者：
Liu Da-wei;Zhang Yu;Kong Wei
本研究建立了由A0X1启动子驱动的重组毕赤酵母表达系统，用于表达HPV16L1蛋白。将HPV16L1基因克隆到载体pPICZαB中，用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法和Western blotting检测甲醇诱导的HPV16L1蛋白的表达。结果表明，重组巴斯德毕赤酵母可分泌HPV16L1蛋白，纯化的HPV16L1蛋白可自组装成病毒样颗粒(VLP)，具有良好的免疫原性，可诱导小鼠产生高效价抗体。
In this study, a recombinant Pichia pastoris expression system was developed to express HPV16 L1 protein that was driven by a strong A0X1 promoter. HPV16L1 gene was cloned into vector pPICZ alpha B. HPV16 L1 protein expression induced by methanol was screened by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. The results indicate that the HPV16 L1 protein is secreted by the recombinant P. pastoris, and the purified HPV16 L1 protein can self-assemble into virus-like particles(VLPs), which show a good immunogenicity and induces high-titer antibody in mice.