Effective Neuroprotection by Ischemic Postconditioning is Associated with a Decreased Expression of RGMa and Inflammation Mediators in Ischemic Rats
Effective Neuroprotection by Ischemic Postconditioning is Associated with a Decreased Expression of RGMa and Inflammation Mediators in Ischemic Rats
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缺血后处理的有效神经保护与缺血大鼠 RGMa 和炎症介质表达的降低有关
DOI：
10.1007/s11064-013-0984-5
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发表时间：
2013-04-01
影响因子：
4.4
通讯作者：
Qin, Xinyue
中科院分区：
文献类型：
--
作者：
Kong, Yuhan;Rogers, Mary R.;Qin, Xinyue
缺血后处理（IPC）能否显着减轻缺血性损伤取决于采取适当的措施。在本研究中，研究了 RGMa 和 IL-1β、IL-6 的表达，以评估脑缺血/再灌注后各种后处理策略的治疗效果。该研究由假手术组和五个治疗组组成：缺血/再灌注（I/R）组、两个邻近缺血后处理组（IPC-S和IPC-M）、远程后处理组（RIPC）和延迟后处理组（DIPC）。我们发现，缺血/再灌注后，IPC 和 RIPC 组的大鼠比 I/R 和 DIPC 组的大鼠表现出明显更少的神经缺陷和更低的梗塞体积。此外，在缺血皮质和海马中，IPC和RIPC组中RGMa的mRNA水平要低得多。免疫组化分析显示，IPC和RIPC组（特别是IPC-S组）RGMa、IL-1β和IL-6的表达降低。此外，神经丝染色显示IPC和RIPC组大鼠的轴突损伤少于I/R和DIPC组。我们的研究表明，减轻脑缺血/再灌注的最佳策略是通过早期、短期和多个周期的近端 IPC 来实现。 IPC的脑保护作用可能与降低RGMa和炎症介质的表达有关。
Whether ischemic postconditioning (IPC) can significantly alleviate ischemic injury hinges on the appropriate measure. In this study, the expression RGMa and IL-1 beta, IL-6 are investigated to estimate the therapeutic benefits of various postconditioning strategies after cerebral ischemia/reperfusion. The study consists of the sham-operated group and five treatment groups: ischemia/reperfusion (I/R), two proximate ischemic postconditioning (IPC-S and IPC-M), remote postconditioning (RIPC) and delayed postconditioning (DIPC) groups. We find that rats in IPC and RIPC groups exhibit significantly less neural deficit and lower infarct volume than that in I/R and DIPC groups after ischemia/reperfusion. Moreover, in ischemic cortex and hippocampus, the mRNA level of RGMa is much lower in IPC and RIPC groups. Immunohistochemical analysis indicates that the expression of RGMa, IL-1 beta and IL-6 are reduced in IPC and RIPC groups (especially in IPC-S group). Furthermore, neurofilament staining reveals that the rats in IPC and RIPC groups have less axonal injury than that in I/R and DIPC groups. Our studies suggest that the optimal strategy to attenuate cerebral ischemia/reperfusion is achieved by early, short-term, and multiple cycles of proximal IPC. The cerebral protective effect of IPC may be associated with the decreased expression of RGMa and inflammation mediators.