In vitro cell cultures as a model of the basal forebrain.
In vitro cell cultures as a model of the basal forebrain.
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作为基底前脑模型的体外细胞培养物。
DOI：
10.1007/978-1-4757-0145-6_24
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发表时间：
1991
影响因子：
--
通讯作者：
Hammond,DN
中科院分区：
文献类型：
--
作者：
Wainer,BH;Lee,HJ;Roback,JD;Hammond,DN
基底前脑因其在学习、记忆和行为状态控制等复杂功能中的假定作用以及在阿尔茨海默氏病（AD）等神经系统疾病中的脆弱性而引起了相当大的关注。神经生长因子为胆碱能基底前脑神经元提供营养支持的发现激发了人们对了解基底前脑神经元的营养相互作用以及疾病状态可能的营养因子治疗策略的进一步兴趣。我们的实验室利用原代细胞培养物并开发了永生化的中枢神经系统细胞系来研究建立和维持中隔海马通路的营养相互作用，中隔海马通路是一种基底前脑成分，在认知功能中发挥着重要作用，在 AD 中受到显着影响。我们的原代细胞培养研究的结果证明了海马体产生的营养信号在介导间隔胆碱能神经元发育中的重要性。神经生长因子在此过程中发挥着重要作用，但它不能解释真正的海马靶细胞产生的所有营养信号。该实验室开发的隔膜和海马谱系克隆细胞系为研究神经营养信号的反应和阐述提供了前景，其分辨率水平比原代培养物更精确。这些细胞系工程产生的技术和信息也将作为一种策略，在未来几年研究其他大脑回路中的营养相互作用，并研究神经系统疾病可能发生的变化或功能障碍。
The basal forebrain has attracted considerable attention because of its putative role in complex functions such as learning, memory and behavioral state control as well as its vulnerability in neurological disorders such as Alzheimer’s Disease (AD). The finding that nerve growth factor provides trophic support for the cholinergic basal forebrain neurons has stimulated further interest in understanding trophic interactions of basal forebrain neurons as well as in possible trophic factor therapeutic stategies for disease states. Our laboratory has utilized primary cell cultures and developed immortalized central nervous system cell lines to study the trophic interactions that establish and maintain the septohippocampal pathway, a basal forebrain component which plays an essential role in cognitive function and is prominently affected in AD. The results of our primary cell culture studies have demonstrated the importance of trophic signals elaborated by the hippocampus in mediating the development of septal cholinergic neurons. Nerve growth factor plays an important role in this process, but it cannot account for all of the trophic signals elaborated by authentic hippocampal target cells. The development by this laboratory of clonal cell lines of septal and hippocampal lineage offers the prospect of investigating both the response to and elaboration of neural trophic signals at a more precise level of resolution than can be achieved with primary cultures. The technology and information that is generated from the engineering of such cell lines will also serve as a strategy to study trophic interactions in other brain circuits in future years, and to investigate possible changes or dysfunctions that occur neurological disease.