Physiological osmolarities do not enhance long-term tissue synthesis in chondrocyte-laden degradable poly(ethylene glycol) hydrogels.
Physiological osmolarities do not enhance long-term tissue synthesis in chondrocyte-laden degradable poly(ethylene glycol) hydrogels.
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生理渗透压不会增强富含软骨细胞的可降解聚乙二醇水凝胶的长期组织合成。
DOI：
10.1002/jbm.a.35329
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Bryant,StephanieJ
中科院分区：
文献类型：
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作者：
Skaalure,StaceyC;Radhakrishnan,SaikripaM;Bryant,StephanieJ
将软骨细胞封装在用于软骨组织工程的合成和可降解水凝胶中可以调节支架降解，但不提供生物线索。概括软骨间质液生理渗透压的培养基可以在短期内增强基质合成，但长期效益仍有待确定。本研究使用从三个骨骼成熟牛供体中分离的软骨细胞，封装在可降解的聚乙二醇水凝胶中，研究了培养基渗透压对组织合成的长期影响。将充满细胞的水凝胶在标准软骨细胞特异性培养基（330 mOsm）或用蔗糖或盐（NaCl 和 KCl）调整的培养基中培养长达 4 周，以达到生理渗透压（400 mOsm）。通过定量和免疫荧光方法评估新软骨基质合成和基质分解代谢。无细胞构建体的水凝胶降解动力学不受介质渗透压或渗透剂的影响。在所有条件下，基质组成主要是聚集蛋白聚糖和 II 型胶原。封装一天后，无论渗透剂如何，在 400 mOsm 培养基中，构建体中积累的总胶原蛋白增加了 80-90%。然而，这种效果并没有持续，4 周时，合成并释放到培养基中的总胶原蛋白在 330 mOsm 培养基中高出三倍多。中等渗透压对硫酸化糖胺聚糖含量影响最小，并且不影响分解代谢活性。这些发现表明，生理渗透压的培养基可能不利于可降解水凝胶中的长期软骨细胞培养，但最初在较高渗透压下培养软骨细胞可能会增强早期组织沉积。 © 2014 Wiley periodicals, Inc. J Biomed Mater Res Part A: 103A: 2186–2192, 2015。
Encapsulating chondrocytes in synthetic and degradable hydrogels for cartilage tissue engineering enables tuning of scaffold degradation, but provides no biological cues. Culture medium that recapitulates the physiological osmolarity of the interstitial fluid in cartilage can enhance matrix synthesis in the short term, but long‐term benefits remain to be determined. This study investigates the long‐term effect of culture medium osmolarity on tissue synthesis using chondrocytes isolated from three skeletally mature bovine donors encapsulated in degradable poly(ethylene glycol) hydrogels. The cell‐laden hydrogels were cultured up to 4 weeks in standard chondrocyte‐specific medium (330 mOsm) or medium adjusted by sucrose or salts (NaCl and KCl) to reach a physiological osmolarity (400 mOsm). Neocartilaginous matrix synthesis and matrix catabolism were evaluated by quantitative and immunofluorescence methods. Hydrogel degradation kinetics of acellular constructs were not affected by medium osmolarity or osmolyte. Matrix composition was predominantly aggrecan and collagen type II for all conditions. One day after encapsulation, total collagen accumulated in the constructs was increased by 80–90% in 400 mOsm medium, regardless of osmolyte. However, this effect did not persist, and at 4 weeks, total collagen synthesized and released to the medium was more than three times higher in 330 mOsm medium. Medium osmolarity had minimal effects on sulfated glycosaminoglycan content and did not affect catabolic activity. These findings suggest that culture medium at physiological osmolarities may not be beneficial for long‐term chondrocyte culture in degradable hydrogels, but that initially culturing chondrocytes at a higher osmolarity may enhance early tissue deposition. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2186–2192, 2015.