Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa
Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa
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DOI：
10.1016/j.cryobiol.2016.03.007
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发表时间：
2016-06-01
期刊：
影响因子：
2.7
通讯作者：
Cavus, Seda
中科院分区：
文献类型：
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作者：
Alcay, Selim;Gokce, Elif;Cavus, Seda
本研究旨在评价不同抗氧化剂含量的冻干蛋黄膨化剂对山羊精子解冻后质量和孵育恢复力的影响。将混合的精液用两步稀释法稀释至最终浓度为1/5（精液/填充剂）的对照和添加抗氧化剂的冻干填充剂（蛋氨酸、半胱胺和丁基羟基甲苯）。采用低渗肿胀试验（HOST）评估精液样本的精子活力、质膜功能完整性、FITC-Pisum sativum凝集素（PSA-FITC）评估受损顶体、末端脱氧核苷酸转移酶介导的dUTP镍端标记（TUNEL）评估DNA完整性。用丙二醛（MDA）浓度分析膜脂过氧化状态。本研究中，添加抗氧化剂的冻干蛋黄膨化剂对山羊精子参数有有益影响。此外，当添加5mm BHT或半胱胺时，我们在解冻后的山羊精液中获得了更高的质量，即使在孵育6小时后。(C) 2016 Elsevier Inc.版权所有。
The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine. (C) 2016 Elsevier Inc. All rights reserved.