Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata, enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes.
Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata, enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes.
复制标题
PtrABF 基因（一种从枳壳中分离出的 bZIP 转录因子）的过表达通过清除 ROS 和调节应激反应基因的表达来增强烟草的脱水和耐旱性
DOI：
10.1186/1471-2229-10-230
复制
发表时间：
2010-10-25
影响因子：
5.3
通讯作者：
Chen XJ
中科院分区：
文献类型：
--
作者：
Huang XS;Liu JH;Chen XJ
背景干旱是影响植物生长发育和作物产量的主要非生物胁迫之一。阿坝响应元件结合因子（ABF）通过调控逆境应答基因的表达在逆境应答中发挥重要作用。Raf. PtrABF的开放阅读框为1347 bp，编码448个氨基酸，与其它植物ABF序列高度同源。PtrABF在酵母细胞中具有转录激活活性，并能与ABRE结合，支持其作为转录因子的作用。脱水、低温和阿坝处理诱导PtrABF的表达。CaMV 35S启动子控制下的PtrABF在转基因烟草中的异位表达增强了对脱水和干旱的耐受性。在脱水和干旱条件下，转基因植株积累的活性氧水平低于野生型，但3种抗氧化酶的活性和表达水平高于野生型。此外，9个胁迫响应基因的稳态mRNA水平编码的功能或调节蛋白被诱导到更高的水平，在转基因株系与或不与干旱stress.ConclusionsPtrABFis一个bZIP转录因子和功能，在干旱胁迫耐受性的正向调制。该基因可能是耐旱植物分子育种的重要候选基因.
BackgroundDrought is one of the major abiotic stresses affecting plant growth, development and crop productivity. ABA responsive element binding factor (ABF) plays an important role in stress responses via regulating the expression of stress-responsive genes.ResultsIn this study, a gene coding for ABF (PtrABF) was isolated fromPoncirus trifoliata(L.) Raf.PtrABFhad a complete open reading frame of 1347 bp, encoding a 448 amino acid peptide, and shared high sequence identities with ABFs from other plants. PtrABF was subcellularly targeted to the nucleus, exhibited transactivation activity in yeast cell and could bind to ABRE, supporting its role as a transcription factor. Expression levels ofPtrABFwere induced by treatments with dehydration, low temperature and ABA. Ectopic expression ofPtrABFunder the control of a CaMV 35S promoter in transgenic tobacco plants enhanced tolerance to both dehydration and drought. Under dehydration and drought conditions, the transgenic plants accumulated lower levels of reactive oxygen species compared with wild type, accompanied by higher activities and expression levels of three antioxidant enzymes. In addition, steady-state mRNA levels of nine stress-responsive genes coding for either functional or regulatory proteins were induced to higher levels in the transgenic lines with or without drought stress.ConclusionsPtrABFis a bZIP transcription factor and functions in positive modulation of drought stress tolerance. It may be an important candidate gene for molecular breeding of drought- tolerant plants.
登录
查看更多内容
DOI：
10.1073/pnas.0505667103
发表时间：
2006-02-07
影响因子：
11.1
作者：
Furihata, T;Maruyama, K;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K
影响因子：
6.9
作者：
Liu, Ji-Hong;Nada, Kazuyoshi;Moriguchi, Takaya
通讯作者：
Moriguchi, Takaya
DOI：
10.1051/agro:2008021
发表时间：
2009-01-01
期刊：
SUSTAINABLE AGRICULTURE
影响因子：
--
作者：
Farooq, M.;Wahid, A.;Basra, S. M. A.
通讯作者：
Basra, S. M. A.
影响因子：
13.8
作者：
Kim, JB;Kang, JY;Kim, SY
通讯作者：
Kim, SY
影响因子：
4.3
作者：
Liang, YC;Chen, Q;Ding, RX
通讯作者：
Ding, RX