Anti-inflammatory effects of ethanol extracts of Chinese propolis and buds from poplar (Populus x canadensis)
Anti-inflammatory effects of ethanol extracts of Chinese propolis and buds from poplar (Populus x canadensis)
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中国蜂胶和杨树芽（Populus x canadensis）乙醇提取物的抗炎作用
DOI：
10.1016/j.jep.2014.05.037
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发表时间：
2014-08-08
影响因子：
5.4
通讯作者：
Hu, Fuliang
中科院分区：
文献类型：
--
作者：
Wang, Kai;Zhang, Jianglin;Hu, Fuliang
民族药理学相关性：蜂胶作为一种民间药物在许多文化中被广泛使用，由于其广泛的生物学特性和药理学活性，特别是其抗炎作用，其潜在的治疗用途正在获得更广泛的认可。尽管越来越多的研究集中在其生物学活性及其植物来源，但对中国植物的研究还不够。本研究旨在研究杨柳科植物加杨芽（Populus x canadensis Moench）的乙醇提取物（EECP）和白杨芽（EEPB）的抗炎活性。采用DPPH自由基清除法和ABTS自由基清除法评价其抗氧化性能。通过评价EECP和EEPB对LPS/IFN-γ共刺激的RAW 264.7细胞中的关键炎性细胞因子和介质的调节作用以及通过使用报告基因测定测量TNF-α或IL-1 β刺激的HEK 293细胞中的核因子（NF）-κ B活化，在体外研究了EECP和EEPB的抗炎作用。结果：EECP和EEPB具有较强的自由基清除活性，并通过调节关键炎症介质的mRNA转录，抑制特异性炎症细胞因子的产生，阻断核因子（NF）-κ B B的活化，发挥显著的体外抗炎作用。EECP和EEPB的管理（25和100 mg/kg）通过减轻肺组织病理学变化和抑制LPS刺激的炎性细胞因子（如白细胞介素-6（IL-6）、IL-10、MCP-1、TNF-α和IL-12 p70）的分泌提供了显著的保护作用。结果表明，毛白杨和杨树芽具有较强的抗炎活性，为开发毛白杨替代品防治炎症性疾病提供了生物学信息。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Ethnopharrnacological relevance: Propolis is used widely in a number of cultures as a folk medicine and is gaining wider recognition for its potential therapeutic use, due to its wide range of biological properties and pharmacological activities, especially its anti-inflammatory effects. Despite an increasing number of studies focused on the biological activities of propolis together with its botanical sources, studies on Chinese propolis are insufficient. This study was designed to investigate the anti-inflammatory properties of ethanol extracts from Chinese propolis (EECP) and poplar buds (EEPB) from Populus x canadensis Moench (Salicaceae family).Materials and methods: Phytochemical analysis of EECP and EEPB was performed via total phenolic and fiavonoid content measurements followed by high-performance liquid chromatography (HPLC) analysis. DPPH and ABTS free-radical scavenging methods were used to evaluate their anti-oxidant properties. The anti-inflammatory effects of EECP and EEPB were investigated in vitro by evaluating their modulating effects on the key inflammatory cytokines and mediators in LPS/IFN-gamma co-stimulated RAW 264.7 cells and by measuring nuclear factor (NF)-kappa B activation in TNF-alpha or IL-1 beta stimulation HEK 293 cells using reporter gene assays. Their effects on acute inflammatory symptoms (LPS-induced endotoxemia and acute pulmonary damage) were also examined in mice.Results: EECP and EEPB exhibited strong free-radical scavenging activity and significant in vitro anti-inflammatory effects by modulating key inflammatory mediators of mRNA transcription, inhibiting the production of specific inflammatory cytokines, and blocking the activation of nuclear factor (NF)-kappa B. The administration of EECP and EEPB (25 and 100 mg/kg) provided significant protective effects by attenuating lung histopathological changes and suppressing the secretion of LPS-stimulated inflammatory cytokines, such as interleukin-6 (IL-6), IL-10, MCP-1, TNF-alpha and IL-12p70 production in endotoxemic mice.Conclusions: The results presented here reveal the potent anti-inflammatory properties of Chinese propolis and poplar buds, and provide biological information for developing suitable substitute(s) for propolis in the prevention and treatment of inflammatory diseases. (C) 2014 Elsevier Ireland Ltd. All rights reserved.