Effects of Panax notoginseng saponin on α, β, and γ secretase involved in Aβ deposition in SAMP8 mice
Effects of Panax notoginseng saponin on α, β, and γ secretase involved in Aβ deposition in SAMP8 mice
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DOI：
10.1097/wnr.0000000000000048
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发表时间：
2014-01-22
期刊：
影响因子：
1.7
通讯作者：
Zhong, Zhenguo
中科院分区：
文献类型：
--
作者：
Huang, Jinlan;Wu, Dengpan;Zhong, Zhenguo
我们以往的研究表明，三七总皂甙(PNS)对阿尔茨海默病学习障碍具有保护作用，抑制A1-40和A1-42的沉积，降低SAMP8大鼠脑内App的含量。在这项研究中，我们旨在研究三七总皂甙对SAMP8小鼠、和参与A代的分泌酶的影响。结果表明，三七总皂苷可能通过上调ADAM9基因的表达水平来增强分泌酶活性。此外，三七总皂甙通过下调BACE1基因表达水平，显著降低BACE1蛋白水平，从而抑制分泌酶活性。但是，三七总皂苷处理并没有改变分泌酶的活性。综上所述，我们的研究表明，PNS调节与分泌相关的蛋白质和基因表达水平，从而增加分泌酶活性和降低分泌酶活性，这可能是PNS阻止A代发生的机制之一。因此，三七总皂甙可能是治疗阿尔茨海默病的一种有前途的药物。(C)2014沃尔特斯·克鲁沃健康垂直酒吧Lippincott Williams&Wilkins。
Our previous studies have shown that Panax notoginseng saponins (PNS) play a protective role in learning dysfunction of Alzheimer's disease, inhibit the deposition of A1-40 and A1-42, and reduce the content of App in the brain of SAMP8. In this study, we aimed to investigate the effects of PNS on , , and secretase involved in A generation in SAMP8 mice. The results showed that PNS increased -secretase activity perhaps by enhancing the level of ADAM9 expression, which itself was achieved by upregulating the expression of the ADAM9 gene. In addition, PNS significantly decreased BACE1 protein level by downregulating the level of BACE1 gene expression and consequently precluded the activity of secretase. However, PNS treatment did not modify -secretase activity. Together, our study shows that PNS modulates the levels of protein and gene expressions involved with and secretase, thereby increasing -secretase activity and reducing -secretase activity, which may be one of the mechanisms of PNS precluding A generation. Accordingly, PNS may be a promising agent for Alzheimer's disease. (C) 2014 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.