Expression of Na(+)-K(+)-ATPase alpha 1- and alpha 3-isoforms in adult and neonatal ferret hearts.
Expression of Na(+)-K(+)-ATPase alpha 1- and alpha 3-isoforms in adult and neonatal ferret hearts.
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Na( )-K( )-ATPase α 1- 和 α 3-亚型在成年和新生儿雪貂心脏中的表达。
DOI：
10.1152/ajpheart.1992.263.5.h1430
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Book,CB
中科院分区：
文献类型：
--
作者：
Ng,YC;Book,CB
我们以前已经证明，在成年雪貂心脏中，Na（+）-K（+）-ATP酶的两种α亚单位亚型，α（+）和α，被表达。α（+）-同种型可包含α 2-或α 3-或两种同种型。本研究进一步表征了α（+）-亚型。雪貂心脏的α（+）-同种型不与α 2特异性单克隆抗体反应，而是与两种不同的α 3特异性多克隆抗体反应。在十二烷基硫酸钠聚丙烯酰胺凝胶电泳中，α（+）-亚型的电泳迁移率比α 2-亚型慢，但与α 3-亚型相似。使用金黄色葡萄球菌V8进行有限的蛋白水解肽作图。然后用α 3特异性抗体对蛋白水解片段进行免疫染色。雪貂心脏α（+）-亚型和大鼠脑α 3-亚型的肽图谱是相同的，雪貂心脏α（+）-亚型和雪貂脑α 3-亚型的肽图谱也是相同的。这些结果表明雪貂心脏的α（+）-亚型是α 3-亚型。在出生后的发育过程中，在成年雪貂心脏中表达的同种型（α 1和α 3）也在新生儿心脏中表达。在成人或新生儿心脏α 2-亚型检测不到。心室和心房组织中同种型的相对丰度不同。与心室组织相比，左心房和右心房组织表达的α 3比α 1少得多。它的结论是，不同于大鼠心脏，α 1-和α 3-异构体在成年雪貂心脏中表达。（250字处删节）
We have demonstrated previously that in adult ferret heart two alpha-subunit isoforms of the Na(+)-K(+)-ATPase, alpha(+) and alpha, are expressed. The alpha(+)-isoform may comprise either alpha 2-, or alpha 3-, or both isoforms. The present studies further characterize the alpha(+)-isoform. The alpha(+)-isoform of ferret heart did not react with an alpha 2-specific monoclonal antibody, but rather with two different alpha 3-specific polyclonal antibodies. Electrophoretic mobility of the alpha(+)-isoform in sodium dodecyl sulfate polyacrylamide gel electrophoresis is slower than that of the alpha 2-isoform, but similar to that of the alpha 3-isoform. Limited proteolytic peptide mapping was performed using Staphylococcus aureus V8. Proteolytic fragments were then immunostained with an alpha 3-specific antibody. The peptide maps of ferret heart alpha(+)-isoform and rat brain alpha 3-isoform were identical, as were those of ferret heart alpha(+)-isoform and ferret brain alpha 3-isoform. These results indicate that the alpha(+)-isoform of ferret heart is an alpha 3-isoform. During postnatal development, the same isoforms expressed in the adult ferret heart (alpha 1 and alpha 3), were also expressed in neonatal heart. In adult or neonatal heart alpha 2-isoform was not detectable. Relative abundances of the isoforms in ventricular and atrial tissues differed. Compared with ventricular tissue, left and right atrial tissues expressed much less alpha 3 than alpha 1. It is concluded that, unlike rat heart, alpha 1- and alpha 3-isoforms are expressed in adult ferret heart.(ABSTRACT TRUNCATED AT 250 WORDS)