The plant-specific G protein ? subunit AGG3 influences organ size and shape in Arabidopsis thaliana
The plant-specific G protein ? subunit AGG3 influences organ size and shape in Arabidopsis thaliana
复制标题
植物特有的 G 蛋白 ?
DOI：
10.1111/j.1469-8137.2012.04083.x
复制
发表时间：
2012-05-01
期刊：
影响因子：
9.4
通讯作者：
Li, Yunhai
中科院分区：
文献类型：
--
作者：
Li, Shengjun;Liu, Yaju;Li, Yunhai
通过细胞增殖和细胞扩张控制器官大小和形状是一个基本的发育过程，但在植物中确定有限器官大小和形状的机制在很大程度上是未知的。分子，遗传，细胞学和生物化学的方法被用来表征拟南芥G蛋白的作用？AGG 3基因在器官生长中的作用。在这里，我们描述A。拟南芥AGG 3，其通过增加细胞增殖期来促进花瓣生长。AGG 3的N-末端区域和C-末端结构域对于AGG 3的功能是必需的。相比之下，对一系列特定结构域缺失的AGG 3衍生物的分析表明，这些结构域中任何一个的缺失都不能完全消除AGG 3的功能。GFP-AGG 3融合蛋白定位于质膜。预测的跨膜结构域在AGG 3的质膜定位中起重要作用。遗传分析表明，AGG 3的作用需要一个功能性G蛋白a亚基（GPA 1）和G蛋白β亚基（AGB 1）。我们的研究结果表明，AGG 3，GPA 1和AGB 1在相同的遗传途径中影响器官的大小和形状。thaliana.
Control of organ size and shape by cell proliferation and cell expansion is a fundamental developmental process, but the mechanisms that set the size and shape of determinate organs are largely unknown in plants. Molecular, genetic, cytological and biochemical approaches were used to characterize the roles of the Arabidopsis thaliana G protein ? subunit (AGG3) gene in organ growth. Here, we describe A. thaliana AGG3, which promotes petal growth by increasing the period of cell proliferation. Both the N-terminal region and the C-terminal domains of AGG3 are necessary for the function of AGG3. By contrast, analysis of a series of AGG3 derivatives with deletions in specific domains showed that the deletion of any of these domains cannot completely abolish the function of AGG3. The GFP-AGG3 fusion protein is localized to the plasma membrane. The predicted transmembrane domain plays an important role in the plasma membrane localization of AGG3. Genetic analyses revealed that AGG3 action requires a functional G protein a subunit (GPA1) and G protein beta subunit (AGB1). Our findings demonstrate that AGG3, GPA1 and AGB1 act in the same genetic pathway to influence organ size and shape in A. thaliana.