Ammonia-oxidizing bacterial communities and shaping factors with different Phanerochaete chrysosporium inoculation regimes during agricultural waste composting
Ammonia-oxidizing bacterial communities and shaping factors with different Phanerochaete chrysosporium inoculation regimes during agricultural waste composting
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农业废弃物堆肥过程中不同黄孢原平革菌接种方案的氨氧化细菌群落和塑造因素
DOI：
10.1039/c6ra04817j
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发表时间：
2016
期刊：
影响因子：
3.9
通讯作者：
Wong Jonathan W. C.
中科院分区：
文献类型：
--
作者：
Zhang Jiachao;Luo Lin;Gao Jun;Peng Qinghui;Huang Hongli;Chen Anwei;Lu Lunhui;Yan Binghua;Wong Jonathan W. C.
研究了农业废弃物堆肥过程中接种黄孢原毛革菌对氨氧化细菌群落的影响。采用定量PCR和变性梯度凝胶电泳技术对不同接种方式下的氨氧化细菌群落进行了研究。结果表明，接种黄孢原毛平革菌对AOB amoA基因丰度有一定的刺激作用.不同接种方式的样品中，不同种类的AOB占优势。线性回归分析表明，氨氧化细菌群落丰度与堆体pH呈显著正相关（P < 0.05）。AOB amoA基因结构与水溶性碳（WSC）（P = 0.002，F = 14.17）和堆温（P = 0.04，F = 2.72）的相关性最好。方差分配分析表明，接种引起的样品性质异质性对细菌amoA基因结构的影响（42.9%，P = 0.006）大于不同接种方式对细菌amoA基因结构的影响（23.6%，P = 0.022）。
This research was conducted to determine the effects of Phanerochaete chrysosporium inoculation on the ammonia-oxidizing bacterial (AOB) communities during agricultural waste composting. AOB communities with different inoculation regimes were investigated by quantitative PCR and denaturing gradient gel electrophoresis. Results showed that P. chrysosporium inoculation imposed certain stimulatory effects on the AOB amoA gene abundance. Samples with different inoculation regimes were dominated by different AOB species. Linear regression analysis indicated that the AOB community abundance had a significant positive correlation with pile pH (P < 0.05). The AOB amoA gene structure was best related to water soluble carbon (WSC) (P = 0.002, F = 14.17) and pile temperature (P = 0.04, F = 2.72). Variance partition analysis suggested that the sample property heterogeneity induced by inoculation imposed a greater impact (42.9%, P = 0.006) on the bacterial amoA gene structure than different inoculation regimes (23.6%, P = 0.022).