Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83
Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83
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DOI：
10.1128/jb.185.18.5591-5601.2003
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发表时间：
2003-09-01
影响因子：
3.2
通讯作者：
Fraser, CM
中科院分区：
文献类型：
--
作者：
Nelson, KE;Fleischmann, RD;Fraser, CM
确定了革兰氏阴性、致病性口腔细菌牙龈卟啉单胞菌菌株W83的完整2，343，479-bp基因组序列，该菌株是牙周病的主要贡献者。全基因组比较分析与其他可用的全基因组序列证实了之间的密切关系，噬细胞黄杆菌类（CFB）门和绿硫细菌。在CF B门中，与牙龈卟啉单胞菌最相似的基因组是多形拟杆菌和B的基因组。脆弱的在CFB门之外，与牙龈卟啉单胞菌最相似的基因组是Chlorobium tepidum的基因组，这支持了之前的系统发育研究，该研究表明Chlorobia和CFB门是相关的，尽管距离很远。菌株W83的基因组分析揭示了一系列与这种口腔病原体的新生物学相关的途径和毒力决定因素。在这些决定因素中，至少有6个假定的血凝素样基因和36个以前未鉴定的肽酶。基因组分析还揭示，牙龈卟啉单胞菌可以代谢一系列氨基酸，并产生许多对人类宿主或人类牙龈组织有毒的代谢终产物，并有助于牙周病的发展。
The complete 2,343,479-bp genome sequence of the gram-negative, pathogenic oral bacterium Porphyromonas gingivalis strain W83, a major contributor to periodontal disease, was determined. Whole-genome comparative analysis with other available complete genome sequences confirms the close relationship between the Cytophaga-Flavobacteria-Bacteroides (CFB) phylum and the green-sulfur bacteria. Within the CFB phyla, the genomes most similar to that of P. gingivalis are those of Bacteroides thetaiotaomicron and B. fragilis. Outside of the CFB phyla the most similar genome to P. gingivalis is that of Chlorobium tepidum, supporting the previous phylogenetic studies that indicated that the Chlorobia and CFB phyla are related, albeit distantly. Genome analysis of strain W83 reveals a range of pathways and virulence determinants that relate to the novel biology of this oral pathogen. Among these determinants are at least six putative hemagglutinin-like genes and 36 previously unidentified peptidases. Genome analysis also reveals that P. gingivalis can metabolize a range of amino acids and generate a number of metabolic end products that are toxic to the human host or human gingival tissue and contribute to the development of periodontal disease.