Gene transfer of hepatocyte growth factor attenuates postinfarction heart failure
Gene transfer of hepatocyte growth factor attenuates postinfarction heart failure
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DOI：
10.1161/01.cir.0000087444.53354.66
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发表时间：
2003-09-09
期刊：
影响因子：
37.8
通讯作者：
Sweeney, HL
中科院分区：
文献类型：
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作者：
Jayasankar, V;Woo, YJ;Sweeney, HL
背景：尽管外科手术和经皮冠状动脉血运重建术取得了进展，但不适合标准血运重建术的持续缺血是导致发病率和死亡率的主要原因。肝细胞生长因子（HGF）具有强大的血管生成和抗凋亡活性，本研究在梗死后心力衰竭大鼠模型中评估了HGF基因转移的功能和生化作用。方法与结果：lewis大鼠结扎左冠状动脉前降支，直接在心内注射编码HGF的复制缺陷重组腺病毒（n=10）或空无病毒（n=9）， 6周后进行动物分析。压力-容积导尿管测量结果显示，与Null对照动物相比，HGF组的最大左室压力（79+/-5 vs 56+/-4 mm Hg）显著保留了收缩功能
Background-Despite advances in surgical and percutaneous coronary revascularization, ongoing ischemia that is not amenable to standard revascularization techniques is a major cause of morbidity and mortality. Hepatocyte Growth Factor (HGF) has potent angiogenic and anti-apoptotic activities, and this study evaluated the functional and biochemical effects of HGF gene transfer in a rat model of postinfarction heart failure.Methods and Results-Lewis rats underwent ligation of the left anterior descending coronary artery with direct intramyocardial injection of replication-deficient recombinant adenovirus encoding HGF (n=10) or empty null virus as control (n=9), and animals were analyzed after six weeks. Pressure-volume conductance catheter measurements demonstrated significantly preserved contractile function in the HGF group compared with Null control animals as measured by maximum developed LV pressure (79+/-5 versus 56+/-4 mm Hg, P