A Highly Efficacious Electrical Biofilm Treatment System for Combating Chronic Wound Bacterial Infections.
A Highly Efficacious Electrical Biofilm Treatment System for Combating Chronic Wound Bacterial Infections.
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用于对抗慢性伤口细菌感染的高效电生物膜治疗系统。
DOI：
10.1002/adma.202208069
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Zhao,Siwei
中科院分区：
文献类型：
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作者：
Zhao,Fan;Su,Yajuan;Wang,Junying;Romanova,Svetlana;DiMaio,DominickJ;Xie,Jingwei;Zhao,Siwei
生物膜感染在慢性伤口中具有高患病率，并且可以延迟伤口愈合。目前使用清创术和抗生素给药的治疗给患者和医疗保健系统带来了巨大的负担。为了解决它们的局限性，本文描述了一种高效的电沉积膜处理系统。该系统使用高强度电流（75 mA cm−2）完全清除创面上方的生物膜，同时增强抗生素向生物膜感染伤口的输送。结合这两种效应，该系统使用短时间治疗（≤2 h）将耐甲氧西林金黄色葡萄球菌（MRSA）生物膜感染的离体皮肤伤口的细菌计数从1010减少到105.2菌落形成单位（CFU）g−1。利用水凝胶离子回路设计，与传统器械相比，该系统增强了高强度电流应用的体内安全性。使用基于糖尿病小鼠的伤口感染模型测试该系统的体内抗菌膜功效。治疗后1天，MRSA生物膜细菌计数从109.0降至104.6CFU g-1，治疗后7天降至103.3CFU g-1，两者均低于临床感染阈值。总的来说，这种新技术为慢性伤口生物膜感染提供了快速、安全、高效的治疗。
Biofilm infection has a high prevalence in chronic wounds and can delay wound healing. Current treatment using debridement and antibiotic administration imposes a significant burden on patients and healthcare systems. To address their limitations, a highly efficacious electrical antibiofilm treatment system is described in this paper. This system uses high‐intensity current (75 mA cm−2) to completely debride biofilm above the wound surface and enhance antibiotic delivery into biofilm‐infected wounds simultaneously. Combining these two effects, this system uses short treatments (≤2 h) to reduce bacterial count of methicillin‐resistantS. aureus(MRSA) biofilm‐infected ex vivo skin wounds from 1010to 105.2colony‐forming units (CFU) g−1. Taking advantage of the hydrogel ionic circuit design, this system enhances the in vivo safety of high‐intensity current application compared to conventional devices. The in vivo antibiofilm efficacy of the system is tested using a diabetic mouse‐based wound infection model. MRSA biofilm bacterial count decreases from 109.0to 104.6CFU g−1at 1 day post‐treatment and to 103.3CFU g−1at 7 days post‐treatment, both of which are below the clinical threshold for infection. Overall, this novel technology provides a quick, safe, yet highly efficacious treatment to chronic wound biofilm infections.