Immunohistochemical study on the distribution of insulin-like growth factor I (IGF-I) receptor in the central nervous system of SOD1G93A mutant transgenic mice
Immunohistochemical study on the distribution of insulin-like growth factor I (IGF-I) receptor in the central nervous system of SOD1G93A mutant transgenic mice
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DOI：
10.1016/j.brainres.2003.09.047
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发表时间：
2003-12-24
期刊：
影响因子：
2.9
通讯作者：
Cha, CI
中科院分区：
文献类型：
--
作者：
Chung, YH;Joo, KM;Cha, CI
在本研究中，我们使用SOD 1（G93 A）突变型转基因小鼠作为ALS的体内模型，并进行免疫组织化学研究，以探讨中枢神经系统胰岛素样生长因子I（IGF-I）受体的变化。在SOD 1（G93 A）转基因小鼠的脊髓、脑干、中央灰质和小脑核中检测到IGF-I受体免疫反应阳性星形胶质细胞。与转基因小鼠相反，在wtSOD 1转基因小鼠的任何脑区均未观察到IGF-I受体免疫反应性星形胶质细胞，但观察到一些中度染色的神经元。在SOD 1（G93 A）转基因小鼠的海马结构中，IGF-I受体免疫反应性在CA 1 -3区的锥体细胞和齿状回的颗粒细胞中增加。本研究首次证明了SODG 93 A转基因小鼠中枢神经系统中反应性星形胶质细胞的IGF-I受体免疫反应性增加，提示反应性星形胶质细胞可能在ALS的发病和进展中起重要作用。IGF-I受体免疫反应性增加的机制以及这些增加的功能意义需要阐明。(C)2003 Elsevier B. V.保留所有权利。
In the present study, we used the SOD1(G93A) mutant transgenic mice as an in vivo model of ALS and performed immunohistochemical studies to investigate the changes of insulin-like growth factor I (IGF-I) receptor in the central nervous system. IGF-I receptor-immunoreactive astrocytes were detected in the spinal cord, brainstem, central gray and cerebellar nuclei of SOD1(G93A) transgenic mice. In contrast to transgenic mice, no IGF-I receptor-immunoreactive astrocytes were observed in any brain region of wtSOD1 transgenic mice although a few moderately stained neurons were observed. In the hippocampal formation of SOD1(G93A) transgenic mice, IGF-I receptor immunoreactivity was increased in the pyramidal cells of the CA1-3 regions and granule cells of the dentate gyrus. The present study provides the first evidence that IGF-I receptor immunoreactivity was increased in reactive astrocytes in the central nervous system of SODG93A transgenic mice, suggesting that reactive astrocytes may play an important role in the pathogenesis and progress of ALS. The mechanisms underlying the increased immunoreactivity for IGF-I receptor, and the functional implications of these increases, require elucidation. (C) 2003 Elsevier B.V. All rights reserved.