Dependence of stem cell fate in Arabidopsis an a feedback loop regulated by CLV3 activity
Dependence of stem cell fate in Arabidopsis an a feedback loop regulated by CLV3 activity
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DOI：
10.1126/science.289.5479.617
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发表时间：
2000-07-28
期刊：
影响因子：
56.9
通讯作者：
Simon, R
中科院分区：
文献类型：
--
作者：
Brand, U;Fletcher, JC;Simon, R
植物分生组织中干细胞的命运受负反馈调控的定向信号系统的支配。在拟南芥（Arabidopsis thaliana）中，CLAVATA （CLV）基因编码负性干细胞限制通路的基本成分。我们使用过表达CLV3的转基因植物来证明分生组织细胞的积累和命运直接取决于CLV3活性的水平，并且CLV3信号通路仅通过CLV1/ CLV2受体激酶复合物发生。我们还证明CLV通路通过抑制转录因子WUSCHEL的活性起作用，WUSCHEL是阳性干细胞促进通路的一个元素。
The fate of stem cells in plant meristems is governed by directional signaling systems that are regulated by negative feedback. In Arabidopsis thaliana, the CLAVATA (CLV) genes encode the essential components of a negative, stem cell-restricting pathway. We used transgenic plants overexpressing CLV3 to show that meristem cell accumulation and fate depends directly on the Level of CLV3 activity and that CLV3 signaling occurs exclusively through a CLV1/ CLV2 receptor kinase complex. We also demonstrate that the CLV pathway acts by repressing the activity of the transcription factor WUSCHEL, an element of the positive, stem cell-promoting pathway.