Targeting and tracing antigens in live cells with fluorescent nanobodies
Targeting and tracing antigens in live cells with fluorescent nanobodies
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DOI：
10.1038/nmeth953
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发表时间：
2006-11-01
期刊：
影响因子：
48
通讯作者：
Leonhardt, Heinrich
中科院分区：
文献类型：
--
作者：
Rothbauer, Ulrich;Zolghadr, Kourosh;Leonhardt, Heinrich
我们将Camelidae sp.重链抗体的表位识别片段与荧光蛋白融合，生成可在活细胞中表达的荧光抗原结合纳米体（chromobodies）。我们证明了在S期和有丝分裂中，染色体体可以识别和追踪不同亚细胞区室中的抗原。由于任何抗原结构都可以通过这种方式在活细胞中进行靶向和追踪，因此染色体体应该能够进行新的功能研究。
We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.