ANTIGEN-SPECIFICITY OF THE OVINE CYTOTOXIC T-LYMPHOCYTE RESPONSE TO BLUETONGUE VIRUS
ANTIGEN-SPECIFICITY OF THE OVINE CYTOTOXIC T-LYMPHOCYTE RESPONSE TO BLUETONGUE VIRUS
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DOI：
10.1016/0165-2427(94)05410-t
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发表时间：
1995-08-01
影响因子：
1.8
通讯作者：
COUPAR, B
中科院分区：
文献类型：
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作者：
ANDREW, M;WHITELEY, P;COUPAR, B
蓝舌病病毒（BTV）是一种由蠓传播的虫媒病毒，可在绵羊中引起严重疾病。病毒中和抗体和细胞毒性T淋巴细胞（CTL）均在保护性免疫中发挥作用。本研究用表达BTV抗原的重组痘苗病毒测定了BTV免疫绵羊CTL的抗原特异性。结果表明，在所研究的绵羊中，血清型特异性外被蛋白VP2和非结构蛋白NS1是CTL的主要免疫原，外被蛋白VP5和NS3是次要免疫原。未检测到VP7（一种主要的基团反应性内层蛋白）特异性CTL。经血清1型免疫的绵羊CTL具有交叉反应性，能够识别其他血清型感染的靶细胞。进一步的研究表明，交叉反应的CTL识别NS1，但不能识别VP2。
Bluetongue virus (BTV), an arbovirus transmitted by midges, can cause serious disease in sheep. Both virus neutralizing antibody and cytotoxic T lymphocytes (CTL) have been shown to have a role in protective immunity. In this study, the antigen specificity of CTL from BTV-immune sheep has been determined using recombinant vaccinia viruses expressing individual BTV antigens. The results show that, in the sheep studied thus far, the serotype-specific outer coat protein, VP2, and the non-structural protein, NS1 are major immunogens for CTL, with VP5 (an outer coat protein) and NS3 being minor immunogens. No VP7 (a major group-reactive inner coat protein) specific CTL were detected. The CTL from sheep immunized with serotype 1 were cross-reactive and able to recognize target cells infected with other BTV serotypes. Further work demonstrated that the cross-reactive CTL recognized NS1, but not VP2.