Time course changes in AQP4 expression patterns in progressive skeletal muscle atrophy during the early stage of denervation
Time course changes in AQP4 expression patterns in progressive skeletal muscle atrophy during the early stage of denervation
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DOI：
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发表时间：
2020-03
影响因子：
1.9
通讯作者：
M. Ishido;Tomohiro Nakamura
中科院分区：
文献类型：
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作者：
M. Ishido;Tomohiro Nakamura
目的：在骨骼肌中，水通道蛋白4（AQP4）主要调节水代谢。虽然AQP4的表达水平在长期失神经支配下降低，但在失神经支配期间，肌肉萎缩的发生与AQP4表达降低的发生之间的关系仍然未知。本研究探讨了失神经支配早期肌肉萎缩开始的时间和AQP4表达减少开始的时间之间的关系。研究方法：将雌性344只大鼠（8周龄）随机分配到对照（C）、坐骨神经去神经后第1天（D1）、坐骨神经去神经后第4天（D4）和坐骨神经去神经后第7天（D7）组（每组n=6）。在每组的胫骨前肌（TA）中，通过Western印迹分析定量一些靶蛋白的表达水平。结果：去神经后第4天，AQP4表达水平明显降低（p<0.05）。此外，AQP4表达水平的下降开始与失神经早期骨骼肌萎缩的时间一致。结论：本研究表明，AQP4表达水平的下降的进展是部分相关的肌肉萎缩的进展，在早期阶段的失神经支配。
Objectives: In the skeletal muscles, water metabolism is mainly regulated by water channel aquaporin 4 (AQP4). Although the expression level of AQP4 was reduced by long-term denervation, during denervation the relationship between muscle atrophy initiation and AQP4 expression decrease initiation remains unknown. The present study examined the relationship between the timing of muscle atrophy initiation and that of AQP4 expression decrease initiation, during the early stage of denervation. Methods: Female 344 rats (8 weeks of age) were randomly assigned to control (C), day 1 post-sciatic denervation (D1), day 4 post- sciatic denervation (D4) and day 7 post- sciatic denervation (D7) groups (n=6 per group). In the tibialis anterior (TA) muscles of each group, the expression levels of some target proteins were quantified by Western blot analysis. Results: The expression level of AQP4 significantly decreased on day 4 post-denervation (p<0.05). Moreover, the beginning of the decrease in AQP4 expression level was concurrent with the timing of muscle atrophy in the skeletal muscles during the early stage of denervation. Conclusions: The present study suggested that the progression of the decrease in the AQP4 expression level is partly related to the progression of muscle atrophy during the early stage of denervation.