ELECTRON-MICROSCOPIC OBSERVATIONS OF GUINEA-PIG SPERMATOZOA PENETRATING EGGS INVITRO
ELECTRON-MICROSCOPIC OBSERVATIONS OF GUINEA-PIG SPERMATOZOA PENETRATING EGGS INVITRO
复制标题
DOI：
10.1111/j.1440-169x.1976.00015.x
复制
发表时间：
1976-01-01
影响因子：
2.5
通讯作者：
YANAGIMACHI, R
中科院分区：
文献类型：
--
作者：
NODA, YD;YANAGIMACHI, R
将体外成熟的豚鼠卵巢卵母细胞与获能的、顶体反应的精子进行体外授精，并检查精子穿过透明带并进入卵细胞质的情况。通过透明带的精子头已经失去了除顶体内膜和赤道段之外的所有顶体成分。人们经常观察到，精子头部周围的透明带材料的纹理是扭曲的，给人的印象是透明带被分开，至少部分地被精子头部穿过透明带所产生的剪切力分开。当卵子从透明带中释放并受精时，顶体反应的精子立即与卵子表面结合并开始与卵子融合；而具有完整顶体的精子却未能做到这一点。卵子质膜和精子质膜在精子头部的中心区域开始融合。精子头部的前半部以吞噬方式被卵子吞噬，而其后半部则通过卵子和精子质膜之间的融合而被纳入卵子中。精子尾部并入卵子是通过精子和卵子质膜之间的融合来实现的。
Guinea pig ovarian oocytes matured in vitro were inseminated in vitro with capacitated, acrosome-reacted spermatozoa and sperm penetration through the zona pellucida and into the egg cytoplasm was examined. Sperm heads passing through the zona pellucida had already lost all their acrosomal elements except for the inner acrosomal membrane and the equatorial segment. It was often observed that the texture of the zona material around the sperm head was distorted, giving the impression that the zona pellucida was parted, at least partially, by a shearing force produced by the sperm head advancing through the zona. When eggs were freed from their zonae pellucidae and inseminated, the acrosome-reacted spermatozoa immediately bound to the egg surfaces and began to fuse with the eggs; whereas the spermatozoa with intact acrosomes failed to do so. Fusion began between the egg plasma membrane and the sperm plasma membrane at the central region of the sperm head. The anterior half of the sperm head was engulfed by the egg in a phagocytic fashion, while its posterior half was incorporated into the egg by a fusion between egg and sperm plasma membranes. Incorporation of the sperm tail into the egg was achieved by fusion between the sperm and egg plasma membranes.