The prognostic value of leucine-rich repeat-containing G-protein (Lgr5) and its impact on clinicopathological features of colorectal cancer
The prognostic value of leucine-rich repeat-containing G-protein (Lgr5) and its impact on clinicopathological features of colorectal cancer
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DOI：
10.1007/s00432-020-03314-7
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发表时间：
2020-07-15
影响因子：
3.6
通讯作者：
Szylberg, Lukasz
中科院分区：
文献类型：
--
作者：
Gzil, Arkadiusz;Zarebska, Izabela;Szylberg, Lukasz
简介 结直肠癌（CRC）是世界上最常见的恶性肿瘤之一。最近的研究表明，癌症干细胞 (CSC) 是结直肠癌中肿瘤细胞恶性行为的原因。本研究旨在确定 CSC 标志物 CD44、DCLK1、Lgr5 和 ANXA2 在 CRC 中的临床意义。材料和方法 这项研究是对从 89 名接受结肠切除术的患者身上采集的组织样本进行的。鉴定并标记具有代表性肿瘤区域的福尔马林固定石蜡包埋的组织块。使用抗CD44、抗LGR5、抗ANXA2和抗DCLK1抗体进行免疫组织化学染色。 H评分系统用于测定CRC细胞的免疫强度。结果 Lgr5 的低表达与淋巴结转移的存在显着相关（p = 0.011），而 Lgr5 的高表达与检查的癌症组织样本中的血管侵犯具有统计学意义（p = 0.027）。此外，Lgr5 表达的高 H 分值与总生存率降低显着相关 (p = 0.043)。结论 我们的结果表明 CSC 标志物 Lgr5 与血管侵犯、淋巴结转移的存在和总体生存率较差之间存在密切关系。 Lgr5 的存在可能是一个不利的预后因素，其在癌组织中的高水平与侵袭性病程有关。该标记还可用于评估治疗的有效性。
Introduction Colorectal cancer (CRC) constitutes one of the most prevalent malignancies in the world. Recent research suggests that cancer stem cells (CSCs) are responsible for tumor cell's malignant behavior in CRC. This study has been designed to determinate clinical implications of CSC markers: CD44, DCLK1, Lgr5, and ANXA2 in CRC. Materials and methods The study was performed on tissue samples which were collected from 89 patients undergoing colectomy. Formalin-fixed paraffin-embedded tissue blocks with representative tumor areas were identified and corded. Immunohistochemical staining was performed using anti-CD44, anti-LGR5, anti-ANXA2, and anti-DCLK1 antibodies. The H-score system was utilized to determine the immunointensity of CRC cells. Results The lower expression of Lgr5 was significantly correlated with the presence of lymph-node metastases (p = 0.011), while high expression of Lgr5 was statistically significant in vascular invasion in examined cancer tissue samples (p = 0.027). Moreover, a high H-score value of Lgr5 expression was significantly related to a reduced overall survival rate (p = 0.043). Conclusion Our results suggest a strong relationship between CSC marker Lgr5 and vascular invasion, presence of lymph-node metastasis, and overall poor survival. The presence of Lgr5 might be an unfavorable prognostic factor, and its high level in cancer tissue is related to an aggressive course. This marker could also be used to access the effectiveness of the treatment.