Apparatus and method to recover the Mueller matrix in bright-field microscopy
Apparatus and method to recover the Mueller matrix in bright-field microscopy
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DOI：
10.1119/5.0081673
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发表时间：
2022-09
影响因子：
0.9
通讯作者：
S. Obando-Vásquez;A. Doblas;C. Trujillo
中科院分区：
文献类型：
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作者：
S. Obando-Vásquez;A. Doblas;C. Trujillo
我们提出了一个为高级物理教学实验室开发的简单实验，用于计算微观样品的穆勒矩阵。 Mueller 矩阵是通过偏振敏感显微镜获取的基于强度的样品图像获得的。该实验需要明场显微镜和标准偏振光学元件，例如线性偏振器和波片。我们提供了实施该设备的实用程序，测量用作样品的线性偏振器的完整穆勒矩阵，并讨论使用我们的设备和方法分析生物样品的可能性。由于设备和方法简单，该实验可以让学生增加关于光偏振的知识并开始光学仪器方面的培训。
We present a simple experiment developed for the advanced physics instructional laboratory to calculate the Mueller matrix of a microscopic sample. The Mueller matrix is obtained from intensity-based images of the sample acquired by a polarization-sensitive microscope. The experiment requires a bright-field microscope and standard polarizing optical components such as linear polarizers and waveplates. We provide a practical procedure for implementing the apparatus, measuring the complete Mueller matrix of linear polarizers used as samples, and discuss the possibility of analyzing biological samples using our apparatus and method. Due to the simplicity of the apparatus and method, this experiment allows students to increase their knowledge about light polarization and initiate their training in optical instrumentation.