Bacteria induce pigment formation in the basidiomycete Serpula lacrymans.
Bacteria induce pigment formation in the basidiomycete Serpula lacrymans.
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DOI：
10.1111/1462-2920.13558
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发表时间：
2016-12
影响因子：
5.1
通讯作者：
J. Tauber;V. Schroeckh;E. Shelest;A. Brakhage;D. Hoffmeister
中科院分区：
文献类型：
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作者：
J. Tauber;V. Schroeckh;E. Shelest;A. Brakhage;D. Hoffmeister
担子菌真菌在生态系统中具有重要的碳循环功能，在化学上具有产生多种小分子的能力。从醌前体atromentin衍生的显色性天然产物，如杂杂酸和天花子素，已被证明在氧化还原循环中起作用。然而，在跨界自然系统的背景下，这些色素仍然是难以捉摸的。在这里，我们与一种普遍存在的陆地细菌（枯草芽孢杆菌、恶臭假单胞菌或伊朗链霉菌）共同培养了模型腐坏性担子菌。对于每一种，都诱导了编码一种非核糖体肽合成酶样酶（atromentin合成酶）和一种转氨酶的基因簇，这两种酶共同产生atromentin。相应地，在共培养过程中，分泌的阿托美汀衍生色素增加，即，变杂、干性、异氧性和阿托美汀酸。从14个醌合成酶基因（其中12个编码在一个簇中）的生物信息学分析中，发现了一个共同的启动子基序，表明许多担子菌的一般调控机制。
Basidiomycete fungi are characterized ecologically for their vital functional role in ecosystem carbon recycling and chemically for their capacity to produce a diverse array of small molecules. Chromophoric natural products derived from the quinone precursor atromentin, such as variegatic acid and involutin, have been shown to function in redox cycling. Yet, in the context of an inter-kingdom natural system these pigments are still elusive. Here, we co-cultured the model saprotrophic basidiomycete Serpula lacrymans with an ubiquitous terrestrial bacterium, either Bacillus subtilis, Pseudomonas putida, or Streptomyces iranensis. For each, there was induction of the gene cluster encoding a non-ribosomal peptide synthetase-like enzyme (atromentin synthetase) and an aminotransferase which together produce atromentin. Correspondingly, during co-culturing there was an increase in secreted atromentin-derived pigments, i.e., variegatic, xerocomic, isoxerocomic, and atromentic acid. Bioinformatic analyses from 14 quinone synthetase genes, twelve of which are encoded in a cluster, identified a common promoter motif indicating a general regulatory mechanism for numerous basidiomycetes.