RNase P from a photosynthetic organelle contains an RNA homologous to the cyanobacterial counterpart
RNase P from a photosynthetic organelle contains an RNA homologous to the cyanobacterial counterpart
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DOI：
10.1006/jmbi.1996.0145
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发表时间：
1996-03-22
影响因子：
5.6
通讯作者：
Schon, A
中科院分区：
文献类型：
--
作者：
Baum, M;Cordier, A;Schon, A
核糖核酸酶P是一种核糖核蛋白酶，在所有的生物体和细胞器的调查，迄今为止，除了叶绿体中没有酶相关的RNA已被检测到。作为一种方法来回答这个问题是否RNA组分存在于RNase P从光合细胞器，我们已经使用了一个基因导向的策略，并寻找RNase P RNA在质体进化的一个假设的中间体，蓝绿藻的蓝色素。我们已经检测到一个351个核苷酸长的RNA类似于蓝藻RNase P RNA，与建议的二级结构，非常类似于细菌的共识。RNA本身没有催化活性，但核酸酶处理会破坏Cyanelle RNase P全酶的活性，表明RNA的重要作用。因此，蓝色素RNase P结合了细菌和真核生物酶的特性，在RNA酶进化中占据中间位置。因此，第一次描述的核糖核酸酶P从光合细胞器的RNA组分可能是一个重要的一步，了解质体核糖核酸酶P的结构和功能。(C)1996年学术出版社
RNase P is a ribonucleoprotein enzyme in all organisms and organelles investigated so far, with the exception of chloroplasts where no enzyme-associated RNA has been detected to date. As an approach to answer the question whether an RNA component is present in RNase P from photosynthetic organelles, we have used a phylogenetically oriented strategy and searched for RNase P RNA in a postulated intermediate in plastid evolution, the cyanelle of Cyanophora paradoxa. We have detected a 351 nucleotide long RNA similar to cyanobacterial RNase P RNAs, with a proposed secondary structure that closely resembles a bacterial consensus. The RNA is encoded on the cyanelle genome and copurifies with enzyme activity The RNA is not catalytically active by itself, but the activity of the cyanelle RNase P holoenzyme is destroyed by nuclease treatment, indicating an essential role of the RNA. Hence cyanelle RNase P combining properties of bacterial and eukaryotic enzymes, occupies an intermediate position in RNA enzyme evolution. The first description of an RNA component in RNase P from a photosynthetic organelle might thus be an important step towards an understanding of plastid RNase P structure and function. (C) 1996 Academic Press Limited