The role of melatonin on gastric mucosal cell proliferation and telomerase activity in ageing
The role of melatonin on gastric mucosal cell proliferation and telomerase activity in ageing
复制标题
DOI：
10.1111/j.1600-079x.2009.00715.x
复制
发表时间：
2009-11-01
影响因子：
10.3
通讯作者：
Akbulut, Hakan
中科院分区：
文献类型：
--
作者：
Akbulut, K. Gonca;Gonul, Bilge;Akbulut, Hakan
尽管褪黑素对培养的肿瘤细胞有抑制增殖的作用，但它对正常细胞的作用尚不清楚。褪黑素在胃粘膜组织老化过程中对端粒酶活性的作用也不清楚。在本研究中，我们研究了胃粘膜端粒酶活性和细胞增殖率随年龄的变化以及褪黑素的影响。将37只青年(4月龄)和老年(20月龄)Wistar大鼠分成对照组(PBS)和老年(20月龄)大鼠。21天]和褪黑素治疗(褪黑素10 mg/kg，i.p.21天)组。测定胃组织端粒酶活性、细胞增殖率、丙二醛(MDA)和谷胱甘肽(GSH)含量。褪黑素对青年和老年大鼠胃黏膜增殖率均有明显的抑制作用。与年轻大鼠相比，老年大鼠的端粒酶活性显著降低。褪黑素显著提高幼年和老年大鼠的端粒酶活性。老年大鼠胃粘膜组织中丙二醛含量显著高于青年大鼠。而老龄大鼠胃粘膜GSH含量显著低于青年大鼠。褪黑素对幼年和老年大鼠的GSH水平均无影响，但显著降低老年动物的丙二醛水平。综上所述，褪黑素可能通过促进端粒酶活性、抑制细胞增殖和脂质过氧化作用而抑制细胞的复制衰老，从而延缓胃粘膜的衰老。
Despite antiproliferative effects of melatonin on cultured tumor cells, its effects on normal cells are less clear. The action of melatonin on telomerase activity in ageing of gastric mucosal tissues also is not known. In this study, we investigated the age-related changes in telomerase activity and cellular proliferation rate of gastric mucosa and the effect of melatonin. A total of 37 young (4 months old), and aged (20 months old) Wistar rats, kept under equal periods of light and dark, were divided into control [(PBS), i.p. for 21 days] and melatonin-treated (10 mg/kg melatonin, i.p. for 21 days) groups. Telomerase activity, cell proliferation rate, malondialdehyde (MDA) and glutathione (GSH) levels of the stomach were determined. Melatonin significantly inhibited the gastric mucosal proliferation rate of both young and aged rats. Telomerase activity was significantly reduced in aged rats compared to young animals. Melatonin significantly increased the telomerase activity of both young and aged rats. The MDA levels of gastric mucosa in the aged rats were significantly higher than those of the younger rats. On the contrary, the GSH levels of gastric mucosa of the aged group were significantly lower than that of the young rats. While melatonin had no effect on GSH levels of either young or aged rats, it significantly decreased the MDA levels in aged animals. In conclusion, melatonin may delay the ageing of gastric mucosa by inhibiting the replicative cellular senescence via its stimulatory effect on telomerase activity and suppressive effect on cellular proliferation and lipid peroxidation.