Transcriptional Regulation of Seven ERFs in Rice Under Oxygen Depletion and Iron Overload Stress
Transcriptional Regulation of Seven ERFs in Rice Under Oxygen Depletion and Iron Overload Stress
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DOI：
10.1007/s12042-013-9117-1
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发表时间：
2013-03-01
影响因子：
2
通讯作者：
de Oliveira, Antonio Costa
中科院分区：
文献类型：
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作者：
dos Santos, Railson Schreinert;Krueger, Mariana Madruga;de Oliveira, Antonio Costa
由于氧气耗尽的压力，淹水会影响植物的发育。淹水的另一个后果是当土壤处于厌氧条件下时，过量的可溶性铁对植物产生毒性。目前，乙烯反应转录因子(ERF)家族的作用受到了广泛关注，尤其是当植物对氧气耗竭做出反应时。考虑到这种情况，我们旨在评估水稻幼苗叶片中7个ERF基因在缺氧(N-2)、低氧(淹水)和铁过载(FeSO4中心点7H(2)O的2000mgL(-1))条件下的转录反应。所有被分析的基因都显示出对这三种胁迫的差异转录表达。对这些转录因子启动子区域的分析表明，最接近转录起始点的启动子区域是这些基因在缺氧(缺氧)或还原(缺氧)条件下的差异反应的最主要原因。另一方面，对于铁胁迫，没有检测到启动子关系。这些区域的甲基化也被认为是一种调节手段，以及在相似的时间和压力条件下表达的不同基因冗余的可能性。
Submergence can affect plant development due to the stress of oxygen depletion. Another consequence of submergence is toxicity to plants by an excess soluble iron when the soil is under anaerobic conditions. Currently, much attention has been given to the role of ethylene response transcription factors (ERFs) family, especially when plants respond to conditions of oxygen depletion. Considering such a scenario, we aimed to evaluate the transcriptional response of seven ERF genes in leaves of rice seedlings subjected to conditions of anoxia (N-2), hypoxia (submergence), and iron overload (2,000 mgL(-1) of FeSO4 center dot 7H(2)O). All the analyzed genes showed differential transcriptional expression in response to these three stresses. Analyses in the promoter region of these transcription factors indicated that the promoter region closest to the transcription start site is most responsible for the differential response of these genes under conditions of oxygen absence (anoxia) or reduction (hypoxia). On the other hand, the promoter relationship was not detected for iron stress. The methylation of these regions as a mean of regulation is also suggested as well as the possibility of redundancy of different genes expressed at similar times and stressful conditions.