The gut microbiota of the pine weevil is similar across Europe and resembles that of other conifer-feeding beetles
The gut microbiota of the pine weevil is similar across Europe and resembles that of other conifer-feeding beetles
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DOI：
10.1111/mec.13702
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发表时间：
2016-08-01
影响因子：
4.9
通讯作者：
Kaltenpoth, Martin
中科院分区：
文献类型：
--
作者：
Berasategui, Aileen;Axelsson, Karolin;Kaltenpoth, Martin
松象甲（Hylobius abictis，鞘翅目：松象甲科）是欧洲针叶树幼苗的重要害虫。尽管其经济的重要性，很少有人知道它的肠道微生物群落的组成和它在介导象鼻虫的能力，利用针叶树作为食物来源的作用。在这里，我们的特点是肠道细菌群落的不同群体的H。并将它们与占据类似生态位的其他甲虫进行了比较。我们证明了H.冷杉属在欧洲各地的较高分类水平（科和属）上相似，沃尔巴克氏体是主要微生物，其次是肠杆菌和厚壁菌门。尽管存在这种相似性，我们还是观察到国家和地点之间存在一致的差异，但性别之间却没有差异。我们的荟萃分析表明，肠道细菌群落的松树象甲是非常相似的小蠹，也利用针叶树作为食物来源。两个宿主类群的肠杆菌科共生体在遗传学上特别密切相关。相反，H.冷杉是不同的密切相关的象鼻虫取食非针叶植物的食物来源，这表明微生物群落的松树象鼻虫是由环境决定的，可能是相关的主机生态。此外，几个H.已知肠杆菌科的冷杉相关成员含有参与萜类降解的基因。因此，我们假设肠道微生物群落对于针叶树幼苗作为食物来源的利用是重要的，无论是通过植物次生代谢产物的解毒还是通过补充必需营养素。
The pine weevil (Hylobius abictis, Coleoptera: Curculionidae) is an important pest of conifer seedlings in Europe. Despite its economic importance, little is known about the composition of its gut microbial community and the role it plays in mediating the weevil's ability to utilize conifers as a food source. Here, we characterized the gut bacterial communities of different populations of H. abietis across Europe and compared them to those of other beetles that occupy similar ecological niches. We demonstrate that the microbial community of H. abietis is similar at higher taxonomic levels (family and genus) across locations in Europe, with Wolbachia as the dominant microbe, followed by Enterobacteria and Firmicutes. Despite this similarity, we observed consistent differences between countries and locations, but not sexes. Our meta-analysis demonstrates that the gut bacterial community of the pine weevil is very similar to that of bark beetles that also exploit conifers as a food source. The Enterobacteriaceae symbionts of both host taxa are especially closely related phylogenetically. Conversely, the microbiota of H. abietis is distinct from that of closely related weevils feeding on nonconifer food sources, suggesting that the microbial community of the pine weevil is determined by the environment and may be relevant to host ecology. Furthermore, several H. abietis-associated members of the Enterobacteriaceae family are known to contain genes involved in terpenoid degradation. As such, we hypothesize that the gut microbial community is important for the utilization of conifer seedlings as a food source, either through the detoxification of plant secondary metabolites or through the supplementation of essential nutrients.