IS50-mediated inverse transposition. Discrimination between the two ends of an IS element.
IS50-mediated inverse transposition. Discrimination between the two ends of an IS element.
复制标题
IS50 介导的逆转座。
DOI：
10.1016/0022-2836(82)90495-8
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发表时间：
1982
影响因子：
5.6
通讯作者：
Berg,DE
中科院分区：
文献类型：
--
作者：
Sasakawa,C;Berg,DE
卡那霉素抗性转座子Tn5的末端由一对几乎相同的插入序列组成，命名为IS50L和IS50R，顺序相反。这些IS50元件的末端由一个极短的(9个碱基对中的8个)反向重复组成。IS50介导的基因从pBR322：：Tn5和相关的细菌质粒转座到噬菌体λ的实验表明，IS50的两端并不等同于转座酶识别的位置。研究发现，IS50的一对外(O)端的使用效率比成对的内(I)端高出三个数量级，但仅略高于One Oend和One Iend。这些数据表明，IS50末端的8个碱基对反向重复序列只包含一个部分转座酶识别位点，而在IS50末端的序列超出了常见的8个碱基对，是有效识别转座酶所必需的。
The termini of the kanamycin resistance transposon Tn5consist of a pair of nearly identical insertion sequences named IS50L and IS50R arranged in inverted orientation. The ends of these IS50elements consist of an extremely short (8 of 9 base-pairs) inverted repeat. Assays of IS50-mediated gene transposition from pBR322:: Tn5and related bacterial plasmids to bacteriophage λ show that the two ends of IS50are not equivalent as sites for transposase recognition. Pairs of outside (O) ends of IS50are found to be used up to three orders of magnitude more efficiently than pairs of inside (I) ends, but only slightly more efficiently than oneOend and oneIend. These data suggest that the eight base-pair inverted repeats at the termini of IS50comprise only a partial transposase recognition site, and that sequences at theOend beyond the common eight base-pairs are needed for efficient transposase recognition.