Structural Characterization and Analysis of Human Immunodeficiency Virus Encapsulating Quantum Dots
Structural Characterization and Analysis of Human Immunodeficiency Virus Encapsulating Quantum Dots
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封装量子点的人类免疫缺陷病毒的结构表征和分析
DOI：
10.1166/jnn.2018.15347
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发表时间：
2018
影响因子：
--
通讯作者：
Cui Zongqiang
中科院分区：
文献类型：
--
作者：
Yin Wen;Li Wei;Li Qin;Qin Chong;Zhang Zhiping;Zhang Xiaowei;Zhang Xian-En;Cui Zongqiang
无机纳米粒子内标记病毒是一种适合于单病毒成像的技术。然而，内部无机纳米颗粒对成熟包膜人免疫缺陷病毒1（HIV-1）病毒核心的形态和结构的影响尚不清楚。此外，完整的HIV-1颗粒和病毒-无机纳米颗粒混合物的分析几乎不可能用常规的阴性染色，因为HIV-1本身是脆弱和不稳定的。在这里，我们进行了形态和结构分析的一个新构建的量子点（QD）封装的感染性HIV-1颗粒通过使用负染色的HIV-1颗粒的电子显微镜分析的优化程序。病毒固定和染色条件进行了优化，以确保HIV-1的完整性，允许随时访问病毒包膜和核心的结构信息。通过优化的TEM进行的形态和结构分析提供了关于病毒、病毒核心和纳米颗粒的关键信息，这表明量子点的包封对病毒颗粒的形态或平均大小，或者对HIV-1核心的形状、长度、宽度或角度没有影响。
Virus internal labeling by inorganic nanoparticle is a suitable technique for single virus imaging. However, it is unknown that the effect of the internal inorganic nanoparticle on morphology and structure of viral core in mature enveloped Human immunodeficiency virus 1 (HIV-1). Also, the analysis of intact HIV-1 particles and virus–inorganic nanoparticle hybrids is almost impossible with conventional negative staining because HIV-1 is inherently fragile and unstable. Herein, we carried out morphological and structural analysis of a newly constructed quantum dot (QD)-encapsulated infectious HIV-1 particle by using an optimized procedure for the electron microscopic analysis of negatively stained HIV-1 particles. The virus fixation and staining conditions were optimized to ensure the integrity of HIV-1, allowing the ready access to structural information on the viral envelope and core. Morphological and structural analysis by optimized TEM provide key information about viruses, viral core and nanoparticles, which indicates that the encapsulation of quantum dots had no effect on the morphology or mean size of the viral particle, or on the shape, length, width, or angle of the HIV-1 core.