The effect of zinc deficiency on alkaline phosphatase (EC 3.1.3.1) and its isoenzymes
The effect of zinc deficiency on alkaline phosphatase (EC 3.1.3.1) and its isoenzymes
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缺锌对碱性磷酸酶（EC 3.1.3.1）及其同工酶的影响
DOI：
10.1079/bjn19800123
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发表时间：
1980
影响因子：
3.6
通讯作者：
F. W. Heaton
中科院分区：
文献类型：
--
作者：
F. A. Adeniyi;F. W. Heaton
1.幼鼠缺锌可降低血清、肾脏、小肠和股骨中总碱性磷酸酶（EC3.1.3.1）活性和锌浓度。2.此外，0.01 mM-外源性锌没有更大的激活效果与锌缺乏大鼠的肾脏，小肠和股骨提取物比对照组，表明缺乏的主要影响是对酶的存在量，而不是其操作的效率。外源性锌使缺锌大鼠血清酶活性升高，但仍低于对照组。3.聚丙烯酰胺凝胶电泳将各组织的碱性磷酸酶活性分离为两条带。这些条带具有相似的电泳迁移率，并且在缺锌大鼠和对照大鼠的相应组织中似乎是定性相同的。4.缺锌消除了对照组大鼠血清中发现的第一条带，并对其他组织中个别带的活性有选择性影响。在肾脏和股骨中，主要抑制酶活性的第一条带，而在小肠中，仅第二条带受到影响。在肝脏中，第一条带的活性增加，第二条带的活性降低了相似的量。
1. Zinc deficiency in young rats reduced both the total alkaline phosphatase (EC 3.1.3.1) activity and Zn concentration in serum, kidney, small intestine and femur. 2. Addition of 0.01 mM-exogenous Zn had no greater activating effect with extracts of kidney, small intestine and femur from Zn-deficient than control rats, indicating that the main effect of the deficiency was on the amount of enzyme present rather than the efficiency of its operation. Exogenous Zn increased the activity of enzyme in serum of Zn-deficient rats, but it was still lower than in the serum of control animals. 3. Electrophoresis on polyacrylamide gel separated the alkaline phosphatase activity from all tissues into two bands. The bands had similar electrophoretic mobilities and appeared to be qualitatively identical in corresponding tissues from Zn-deficient and control rats. 4. Zn deficiency eliminated the first band found in serum from control rats and it had selective effects on the activity of individual bands in other tissues. The major inhibitory effect was on the first bands of enzyme activity in kidney and femur, but in small intestine only the second band was affected. In liver the activity of the first band was increased and that of the second band decreased by similar amounts.