Morphological characteristics and electrophysiological properties of CA1 pyramidal neurons in macaque monkeys
Morphological characteristics and electrophysiological properties of CA1 pyramidal neurons in macaque monkeys
复制标题
DOI：
10.1016/j.neuroscience.2005.07.001
复制
发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Amaral, DG
中科院分区：
文献类型：
--
作者：
Altemus, KL;Lavenex, P;Amaral, DG
非人灵长类动物海马区单个神经元的形态特征和内在电生理特性知之甚少。我们在体外海马片制备中使用了细胞内记录和生物细胞素标记技术，以定量评估猕猴CA1锥体神经元的基本形态和内在电生理特征。这些神经元之前已经在我们实验室的大鼠身上进行了研究。猴CA1锥体神经元胞体平均体积为3578µm(3)，4.71个基树突含53个末梢分支，树突长度约10,164微米，1.13个顶端树突含47个终支，树突长度约10,678微米。与之相比，大鼠CA1锥体神经元胞体平均体积为2066微米(3)，基突3.35个，终支29个，树突长度约4,586微米，顶突1.43个，终支62个，树突长度约8,838微米。猴CA1区锥体神经元的静息膜电位约为-62 mV(大鼠：-62 mV)，输入电阻为35M欧米加(大鼠：34-49M欧米加)，流变基为0.17nA(大鼠：0.12-0.20nA)，动作电位幅度为83 mV(大鼠：71-89 mV)。虽然形态上的差异，如树突长度的增加，可能会转化为灵长类和啮齿动物之间在神经处理方面的差异，但这些形态差异的功能意义尚不清楚。灵长类动物大脑的定量研究对于推断来自啮齿动物研究的信息以更好地了解人类海马体的正常和病理功能是至关重要的。(C)2005年由爱思唯尔有限公司代表IBRO出版。
Little is known about the morphological characteristics and intrinsic electrophysiological properties of individual neurons in the nonhuman primate hippocampus. We have used intracellular recording and biocytin-labeling techniques in the in vitro hippocampal slice preparation to provide quantitative evaluation of the fundamental morphological and intrinsic electrophysiological characteristics of macaque monkey CA1 pyramidal neurons. These neurons have previously been studied in the rat in our laboratory. Monkey CA1 pyramidal neurons have an average soma volume of 3578 mu m(3), 4.71 basal dendrites with 53 terminal branches for a dendritic length of about 10,164 gm, 1.13 apical dendrites with 47 terminal branches for a dendritic length of about 10,678 mu m. In comparison, rat CA1 pyramidal neurons have an average soma volume of 2066 mu m(3), 3.35 basal dendrites with 29 terminal branches for a dendritic length of about 4,586 mu m, 1.43 apical dendrites with 62 terminal branches for a dendritic length of about 8,838 mu m. The basic intrinsic electrophysiological properties of CA1 pyramidal cells are similar in monkeys and rats. Monkey CA1 pyramidal neurons have a resting membrane potential of about -62 mV (rat: -62 mV), an input resistance of 35 M Omega (rat: 34-49 M Omega), a rheobase of 0.17 nA (rat: 0.12-0.20 nA) and an action potential amplitude of 83 mV (rat: 71-89 mV). Although morphological differences such as the increased dendritic length may translate into differences in neural processing between primates and rodents, the functional significance of these morphological differences is not yet clear. Quantitative studies of the primate brain are critical in order to extrapolate information derived from rodent studies into better understanding of the normal and pathological function of the human hippocampus. (c) 2005 Published by Elsevier Ltd on behalf of IBRO.