Long-term in vivo pineal microdialysis.
Long-term in vivo pineal microdialysis.
复制标题
长期体内松果体微透析。
DOI：
10.1034/j.1600-079x.2003.00064.x
复制
发表时间：
2003
影响因子：
10.3
通讯作者：
Borjigin,Jimo
中科院分区：
文献类型：
--
作者：
Sun,Xing;Liu,Tiecheng;Deng,Jie;Borjigin,Jimo
本研究描述了一种长期测量自由活动大鼠松果体中每日5 -羟色胺（5 - HT）和褪黑素含量的新技术的发展。与以前的协议相比，该技术具有许多新颖的改进。它允许可视化松果体的精确定位引导插管，从而最大限度地减少出血；对周围脑组织无直接损伤；不会干扰颈上神经节的交感神经支配。褪黑素和吲哚前体的强劲释放在同一动物中连续定量和可重复地监测超过2周。此外，随着时间的推移，药物对体内松果体昼夜节律的影响可以被重复研究，基因表达谱可以与单个动物的生理后果相关联。通过这些方法，研究人员发现β -肾上腺素能激活导致5 - HT释放减少，而体内cAMP信号的增加导致N -乙酰转移酶基因诱导和褪黑激素产生的激活。这些研究将加强对调节松果体5‐HT和褪黑激素合成和分泌的信号通路的理解。
This study describes the development of a new technique for long‐term measurement of daily 5‐hydroxytryptamine (5‐HT) and melatonin contents in the pineal gland of freely moving rats. The technique features a number of novel improvements over previous protocols. It allows visualization of the pineal gland for accurate targeting of the guide cannula, which minimizes bleeding; incurs no direct injury to the surrounding brain tissues; and causes no interference with the sympathetic innervation from the superior cervical ganglia. Robust releases of melatonin and indole precursors were continuously monitored quantitatively and reproducibly for more than 2 wk in the same animal. In addition, effects of pharmacological agents on in vivo pineal circadian rhythms can be studied reproducibly over time, and gene expression profiles can be correlated with physiological consequences in single animals. Using these approaches, it is found thatβ‐adrenergic activation leads to decreased release of 5‐HT, and that increased cAMP signaling in vivo results in activation of N‐acetyltransferase gene induction and melatonin production. These studies will enhance the understanding of signaling pathways that regulate pineal 5‐HT and melatonin synthesis and secretion.