AN IMMUNOHISTOCHEMICAL INVESTIGATION OF PORCINE EPIDEMIC DIARRHEA
AN IMMUNOHISTOCHEMICAL INVESTIGATION OF PORCINE EPIDEMIC DIARRHEA
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DOI：
10.1016/s0021-9975(05)80069-6
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发表时间：
1995-07-01
影响因子：
0.8
通讯作者：
MORI, M
中科院分区：
文献类型：
--
作者：
SUEYOSHI, M;TSUDA, T;MORI, M
日本发生了仔猪流行性腹泻的突然暴发，主要特征是水样腹泻、脱水和新生动物死亡率高。镜下病变表现为小肠绒毛状萎缩，绒毛状肠细胞呈空泡状，呈立方体状。绒毛与隐窝的比例严重降低，从1:1到3:1不等。透射电镜显示在肠细胞细胞质内和微绒毛间有大量冠状病毒。采用链亲和素-生物素（SAB）技术在肠细胞细胞质中检测出猪流行性腹泻（FED）病毒特异性抗原。感染细胞在空肠和回肠的绒毛上皮中最丰富，在大肠、隐窝上皮、固有层和Peyer's斑块中也有少量感染细胞。本研究表明，SAB技术在诊断胃食管炎方面是有用的。(C) 1995学术出版社有限公司
A sudden outbreak of epidemic diarrhoea of piglets occurred in Japan, the principal features being watery diarrhoea, dehydration and high mortality in newborn animals. The microscopical lesions were villous atrophy in the small intestine, the villous enterocytes being vacuolated and cuboidal in shape. The villus-crypt ratio was severely reduced, varying from 1:1 to 3:1. Transmission electron microscopy showed numerous coronaviruses within the cytoplasm of enterocytes and among microvilli. Specific antigens of porcine epidemic diarrhoea (FED) virus were detected in the cytoplasm of enterocytes by the streptavidin-biotin (SAB) technique. Infected cells, which were most abundant in the villous epithelia of the jejunum and ileum, were present in small numbers in the large intestine, the crypt epithelia, the lamina propria and Peyer's patches. The study suggests that the SAB technique is useful for the diagnosis of FED. (C) 1995 Academic Press Limited