Osteoblast growth on the surface of porous Zn-containing HA/TiO2 hybrid coatings on Ti substrate by MAO plus sol-gel methods
Osteoblast growth on the surface of porous Zn-containing HA/TiO2 hybrid coatings on Ti substrate by MAO plus sol-gel methods
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DOI：
10.1016/j.surfcoat.2012.06.028
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发表时间：
2013-08-15
影响因子：
5.4
通讯作者：
Qi, M.
中科院分区：
文献类型：
--
作者：
Zhang, J. Y.;Ai, H. J.;Qi, M.
为改善钛基材表面羟基磷灰石涂层的骨结合性能，采用溶胶-凝胶法在钛表面制备了含锌羟基磷灰石/二氧化钛杂化涂层，并在钛表面制备了多孔二氧化钛涂层。用电感耦合等离子体光谱仪测量了锌离子的释放浓度。体外共培养实验检测MG-63细胞在多孔表面的形态、附着和骨相关转录因子基因的表达。扫描电子显微镜观察发现，与HA/Ti02涂层(圆形)相比，ZnHA/TiO2光催化涂层的晶胞形貌更呈多边形。激光共聚焦扫描显微镜观察证实，MG-63细胞在杂化涂层上黏附良好。核心区结合转录因子FATATAL和OSTRIX基因的表达增强。结果表明，在钛种植体表面制备的复合涂层具有良好的生物活性。(C)2012爱思唯尔B.V.保留所有权利。
To improve the osseointegration properties for the hydroxyapatite coated titanium substrate, Zn containing hydroxyapatite titania (ZnHA/TiO2) hybrid coatings were prepared on titanium via sol-gel method after micro-arc oxidation which produced a porous titania coating on titanium. The released concentration of Zn2+ was measured by an inductively coupled plasma spectrometer. In vitro co-culture assays were performed to evaluate the morphology, attachment and bone-associated transcription factor gene expression of MG-63 cells on the porous surfaces. The cell micro morphologies on the ZnHA/TiO2 coatings were more polygonal compared with that on HA/TiO2 coatings (round) by SEM. It was confirmed by the confocal laser scanning microscopy observations that the MG-63 cells on the hybrid coating layer were adhered favorably. The expression of transcription factor genes of core binding fatoral and osterix improved on the ZnHA/TiO2 coatings. The results suggested that the ZnHA/TiO2 hybrid coatings on titanium implants possess good bioactivity. (C) 2012 Elsevier B.V. All rights reserved.