Studies on a quantitative analysis of oryzalides and oryzalic acids in rice plants by GC-SIM
Studies on a quantitative analysis of oryzalides and oryzalic acids in rice plants by GC-SIM
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DOI：
10.1271/bbb.60.1460
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发表时间：
1996-09-01
影响因子：
1.6
通讯作者：
Watanabe, M
中科院分区：
文献类型：
--
作者：
Watanabe, M;Kono, Y;Watanabe, M
以17-氘代稻磺内酯B为内标，建立了水稻叶片中稻磺内酯和稻磺酸的GC-MS-选择离子监测（GC-SIM）定量分析方法。结果表明，在不同生育期，苗期、分蘖期和成熟期分别为5.3、17.9和37.9 μ g/g。各器官中谷氨酰胺和谷氨酰胺酸的含量，叶、茎、穗和根的含量分别为35.0和1.7 μ g/g，后两者未检测到。野油菜黄单胞菌致病变种（Xanthomonascampestrispv.）水稻表现出更大的积累oryzalides和oryzalic酸，特别是在病变区，比健康叶片的数字。针束造成的机械损伤也增加了总量。
A procedure for the quantitative analysis of oryzalides and oryzalic acids in leaves of rice cultivars was established by using GC-MS-selective ion monitoring (GC-SIM), 17-deuterated oryzalide B being used as an internal reference. The total contents of four compounds of oryzalides and oryzalic acids in the leaves at three different growth stages of Norin-27, i.e., seedling, tillering, and ripening, showed values of 5.3, 17.9, and 37.9 mu g/g, respectively. The contents of oryzalides and oryzalic acids in each organ, i.e., leaf, stem, ear, and root, showed values of 35.0 and 1.7 mu g/g for the former two, respectively, and were not detectable for the latter two. A preliminary analysis of Norin-27 and Rantai emas leaves inoculated with an incompatible and compatible strain of Xanthomonas campestris pv. oryzae showed a greater accumulation of oryzalides and oryzalic acids, especially in the lesion area, than the figures for healthy leaves. Mechanical injury by a needle-bundle also increased the total amounts.