THE DROSOPHILA ORB RNA-BINDING PROTEIN IS REQUIRED FOR THE FORMATION OF THE EGG CHAMBER AND ESTABLISHMENT OF POLARITY
THE DROSOPHILA ORB RNA-BINDING PROTEIN IS REQUIRED FOR THE FORMATION OF THE EGG CHAMBER AND ESTABLISHMENT OF POLARITY
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DOI：
10.1101/gad.8.5.598
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发表时间：
1994-03-01
影响因子：
10.5
通讯作者：
SCHEDL, P
中科院分区：
文献类型：
--
作者：
LANTZ, V;CHANG, JS;SCHEDL, P
果蝇的球形基因编码含有两个rrm型rna结合域的性别特异性种系蛋白。在此，我们报告了Orb蛋白在野生型、肿瘤型和Orb突变型卵巢中的分布。Orb蛋白在卵发生过程中的野生型分布与其RNA相似，在16细胞囊肿形成后不久优先积聚在发育中的卵母细胞的细胞质中。正如其种系表达所预测的那样，orb的突变导致雌性不育。对orb突变体对已知的卵母细胞分化和极性所需的RNA分布的影响的分析表明，orb在卵发生过程中多个点的RNA定位中起作用。此外，球形突变体的表型特征表明，该基因在卵子形成早期是16细胞囊肿形成所必需的。然后，它在卵母细胞的分化中起作用，是囊肿中生殖细胞三维重组以及在卵腔中建立正常的种系-体细胞相互作用所必需的。
The orb gene of Drosophila encodes sex-specific germ-line proteins that contain two RRM-type RNA-binding domains. Here we report the distribution of Orb protein in wild-type, tumorous, and orb mutant ovaries. The wild-type distribution of Orb protein during oogenesis resembles that of its RNA, preferentially accumulating in the cytoplasm of the developing oocyte shortly after the formation of the 16-cell cyst. As anticipated from its germ-line expression, mutations in orb lead to female sterility. Analysis of the effect of orb mutants on the distribution of RNAs known to be required for oocyte differentiation and polarity suggests that orb functions in RNA localization at multiple points during oogenesis. In addition, phenotypic characterization of the orb mutants indicates that the gene is required early in oogenesis for formation of the 16-cell cyst. It then functions in the differentiation of the oocyte and is required for the three-dimensional reorganization of the germ cells in the cyst as well as for the establishment of normal germ-line-soma interactions in the egg chamber.