Quantified postsurgical small cell size CFCs and EpCAM+ circulating tumor stem cells with cytogenetic abnormalities in hepatocellular carcinoma patients determine cancer relapse
Quantified postsurgical small cell size CFCs and EpCAM+ circulating tumor stem cells with cytogenetic abnormalities in hepatocellular carcinoma patients determine cancer relapse
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DOI：
10.1016/j.canlet.2017.10.004
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发表时间：
2018-01-01
期刊：
影响因子：
9.7
通讯作者：
Dong, Jiahong
中科院分区：
文献类型：
--
作者：
Wang, Liang;Li, Yilin;Dong, Jiahong
用常规策略进行的肝细胞癌循环肿瘤细胞的检测由于EpCAM的固有异质性和动态表达以及上皮-间充质转化期间细胞角蛋白的降解而受到显著限制，这不可避免地导致这种“不可捕获和不可见”CTC的不可忽略的假阴性检测。本研究采用了一种新的SE-iFISH策略，对HCC CTC的检测进行了改进，用于全面检测接受手术切除的患者中肝细胞癌和胆管癌CTC（CD 45（-）/CD 31（-））的原位表型和核型特征。系统研究CTC不同亚型的临床意义。小细胞大小的CFC（= 5个细胞/6 ml血液）、多倍体（>=五体8）CTSC或CTM（任一个>= 1）的存在与HCC患者的不良预后显著相关，表明在术后患者中检测CFC和CTSC的那些特定亚型有助于预测肿瘤复发。(C)2017作者由爱思唯尔公司出版
Detection of hepatocellular carcinoma circulating tumor cells performed with conventional strategies, is significantly limited due to inherently heterogeneous and dynamic expression of EpCAM, as well as degradation of cytokeratins during epithelial-to-mesenchymal transition, which inevitably lead to non negligible false negative detection of such "uncapturable and invisible" CTCs. A novel SE-iFISH strategy, improved for detection of HCC CTCs in this study, was applied to comprehensively detect, in situ phenotypically and karyotypically characterize hepatocellular and cholangiocarcinoma CTCs (CD45(-)/CD31(-)) in patients subjected to surgical resection. Clinical significance of diverse subtypes of CTC was systematically investigated. Existence of small cell size CFCs (= 5 cells/6 ml blood), multiploid (>= pentasomy 8) CTSCs or CTM (either one >= 1) significantly correlated to HCC patients' poor prognosis, indicating that detection of those specific subtypes of CFCs and CTSCs in post-operative patients help predict neoplasm recurrence. (C) 2017 The Authors. Published by Elsevier B.V.