Minimum inhibitory concentrations of amphotericin B, azoles and caspofungin against Candida species are reduced by farnesol
Minimum inhibitory concentrations of amphotericin B, azoles and caspofungin against Candida species are reduced by farnesol
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DOI：
10.3109/13693786.2012.692489
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发表时间：
2013-01-01
期刊：
影响因子：
2.9
通讯作者：
Rocha, Marcos F. G.
中科院分区：
文献类型：
--
作者：
Cordeiro, Rossana A.;Teixeira, Carlos E. C.;Rocha, Marcos F. G.
本研究的目的是评估法尼醇的抗真菌活性及其与传统抗真菌药物对念珠菌耐药菌株的相互作用。为此，我们研究了阿替霉素B（AMB）、氟康唑（FLC）、伊曲康唑（ITC）、卡泊芬净（CAS）和法尼醇对45株念珠菌的体外最低抑菌浓度（MIC），也就是说，24 C.白色念珠菌（C. albicans）16株。近平滑和5 C.通过使用肉汤微量稀释法对热带假单胞菌进行鉴定。然后，用法尼醇加上以前发现耐药的药物组合对分离株进行测试。此外，将菌株在亚抑制法尼醇浓度下预孵育，并重新评估其抗真菌能力。我们发现法尼醇的MIC值在4.68-150 μ M之间变化，MIC > 1 mg/l的19株，MIC ≥ 64 mg/l的18株，MIC ≥ 1 mg/l的35株，MIC ≥ 2 mg/l的6株，或分别对AMB、FLC、ITC和CAS耐药。法尼醇与抗真菌药物联合用药时，以及法尼醇与念珠菌共同孵育时，其MIC均显著降低（P < 0.05）。我们的结论是，法尼醇的体外作用提高了念珠菌对传统抗真菌药物的活性。分离物具有抗性。这些结果支持进一步研究法尼醇在甾醇生物合成途径平衡中的作用以及它如何干扰细胞活力。
The objective of this study was to evaluate the antifungal activity of farnesol and its interaction with traditional antifungals against drug-resistant strains of Candida species. To do so, we studied the minimum in vitro inhibitory concentration (MIC) of amphotericin B (AMB), fluconazole (FLC), itraconazole (ITC), caspofungin (CAS) and farnesol against 45 isolates of Candida spp., i.e., 24 C. albicans, 16 C. parapsilosis and 5 C. tropicalis through the use of the broth microdilution method. Then, the isolates were tested with the combination of farnesol plus drugs to which they were previously found to be resistant. Additionally, the strains were pre-incubated at sub-inhibitory farnesol concentrations and their antifungal susceptibilities were re-evaluated. We found the MIC values for farnesol varied from 4.68-150 mu M for Candida spp., with 19 isolates having a MIC > 1 mg/l, 18 a MIC >= 64 mg/l, 35 having a MIC >= 1 mg/l and 6 isolates a MIC >= 2 mg/l or were resistant to AMB, FLC, ITC and CAS, respectively. Significant MIC reductions were observed when farnesol and antifungal drugs were combined (P < 0.05) and when Candida strains were incubated with farnesol (P < 0.05). We conclude that the in vitro effects of farnesol improved the activity of traditional antifungals to which the Candida spp. isolates were resistant. These results support further investigation of the role of farnesol in the balance of the sterol biosynthetic pathway and how it interferes with cell viability.