Lymphocyte subsets in alcoholic liver disease
Lymphocyte subsets in alcoholic liver disease
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DOI：
10.4254/wjh.v5.i2.46
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发表时间：
2013-02-27
影响因子：
2.4
通讯作者：
Carvalho, Armando
中科院分区：
文献类型：
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作者：
Matos, Luis Costa;Batista, Paulo;Carvalho, Armando
目的：比较淋巴细胞亚群之间的健康对照和酗酒者与liverdisease.METHODS：本研究的患者队列包括个人谁被怀疑有酒精性肝病（ALD），谁进行了肝活检（疾病分级和分期，怀疑诊断，或并发肝病; n = 56）。正常对照组包括因非复杂性胆结石而接受择期胆囊切除术的患者（n = 27）。将福尔马林固定、石蜡包埋的肝活检标本切片，并用苏木精、伊红和珀尔斯普鲁士蓝染色。非酒精性脂肪性肝炎评分用于评估ALD标志物。流式细胞术测定淋巴细胞亚群。鉴定T淋巴细胞（CD 3（+）），然后进一步细分为CD 4（+）或CD 8（+）群体。还测量了B淋巴细胞（CD 19（+））和自然杀伤（NK）细胞数量。除了评估淋巴细胞亚群ALD患者和对照组之间的差异，我们还比较了酒精性肝硬化或禁欲患者正常controls.RESULTS：患者队列主要由老年男性的子集。66.1%的患者存在活动性酒精中毒。报告的平均每日酒精摄入量为164.9 g，平均终生累积摄入量为2211.6 kg。39.3%的患者存在肝硬化，66.1%的患者在其肝脏样本中存在显著的纤维化（窦周和门静脉/门静脉周围纤维化、桥接纤维化或肝硬化）。平均马约终末期肝病评分为7.6分。未发现遗传性血色素沉着症基因型。ALD患者（n = 56）表现为明显的淋巴细胞减少（1.5 x 10（9）/L +/- 0.5 x 10（9）/L vs 2.1 x 10（9）/L +/- 0.5 x 10（9）/L，P < 0.0001），由于除NK淋巴细胞外的所有淋巴细胞亚群减少：CD3（+）（1013.0 +/- 406.2/mm（3）vs 1523.0 +/- 364.6/mm（3），P < 0.0001），CD4（+）（713.5 ± 284.7/mm（3）vs 992.4 ± 274.7/mm（3），P < 0.0001），CD8（+）（262.3 ± 140.4/mm（3）vs 478.9 ± 164.6/mm（3）vs 1523.0 ± 364.6/mm（3），p
AIM: To compare lymphocyte subsets between healthy controls and alcoholics with liver disease.METHODS: The patient cohort for this study included individuals who were suspected to have alcoholic liver disease (ALD) and who had undergone liver biopsy (for disease grading and staging, doubts about diagnosis, or concurrent liver disease; n = 56). Normal controls included patients who were admitted for elective cholecystectomy due to non-complicated gallstones (n = 27). Formalin-fixed, paraffin-embedded liver biopsy specimens were sectioned and stained with hematoxylin and eosin and Perls' Prussian blue. The non-alcoholic steatohepatitis score was used to assess markers of ALD. Lymphocyte population subsets were determined by flow cytometry. T lymphocytes were identified (CD3(+)),and then further subdivided into CD4(+) or CD8(+) populations. B lymphocytes (CD19(+)) and natural killer (NK) cell numbers were also measured. In addition to assessing lymphocyte subpopulation differences between ALD patients and controls, we also compared subsets of alcoholic patients without cirrhosis or abstinent cirrhotic patients to normal controls.RESULTS: The patient cohort primarily consisted of older men. Active alcoholism was present in 66.1%. Reported average daily alcohol intake was 164.9 g and the average lifetime cumulative intake was 2211.6 kg. Cirrhosis was present in 39.3% of the patients and 66.1% had significant fibrosis (perisinusoidal and portal/periportal fibrosis, bridging fibrosis, or cirrhosis) in their liver samples. The average Mayo end-stage liver disease score was 7.6. No hereditary hemochromatosis genotypes were found. ALD patients (n = 56) presented with significant lymphopenia (1.5 x 10(9)/L +/- 0.5 x 10(9)/L vs 2.1 x 10(9)/L +/- 0.5 x 10(9)/L, P < 0.0001), due to a decrease in all lymphocyte subpopulations, except for NK lymphocytes: CD3(+) (1013.0 +/- 406.2/mm(3) vs 1523.0 +/- 364.6/mm(3) , P < 0.0001), CD4(+) (713.5 +/- 284.7/mm(3) vs 992.4 +/- 274.7/mm(3) , P < 0.0001), CD8(+) (262.3 +/- 140.4/mm(3) vs 478.9 +/- 164.6/mm(3 )vs 1523.0 +/- 364.6/mm(3), p