ASSEMBLY OF STRATUM-CORNEUM BASIC-PROTEIN AND KERATIN FILAMENTS IN MACROFIBRILS
ASSEMBLY OF STRATUM-CORNEUM BASIC-PROTEIN AND KERATIN FILAMENTS IN MACROFIBRILS
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DOI：
10.1038/276729a0
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发表时间：
1978-01-01
期刊：
影响因子：
64.8
通讯作者：
STEINERT, PM
中科院分区：
文献类型：
--
作者：
DALE, BA;HOLBROOK, KA;STEINERT, PM
角质层是哺乳动物表皮的最表层，由几层完全分化的死亡细胞组成，这些细胞充满了一种叫做角蛋白的不溶性蛋白质复合物。角质层细胞的早期超微结构研究显示，在深染色的亲锇无定形基质中嵌有一种直径为70-80 Å的浅色细丝的“角蛋白模式”。这些丝在活细胞的内部层合成，被称为张力丝、α-角蛋白，或者更准确地说，角蛋白丝。用解离试剂从牛2 - 4、大鼠5 - 6、小鼠7和人表皮中提取了活细胞和角质层纤维的亚基。在适当的条件下，这些亚基将在体外聚合成直径为uniformally70-80Å的天然型角蛋白丝7,9。相比之下，无定形基质尚未在生化水平上得到肯定的鉴定，尽管最近有人提出，富含组氨酸的角质层碱性蛋白（SCBP）具有与纤维间基质物质可能的功能一致的特性10。我们在这里展示了高度有序的结构，让人想起完整角质层中角蛋白的模式，当体外培养时，SCBP和表皮角蛋白丝结合在一起形成。
THE stratum corneum is the most superficial aspect of mammalian epidermis and consists of several layers of fully differentiated, dead cells filled with an insoluble protein complex called keratin. Early ultrastructural studies of the stratum corneum cells revealed a ‘keratin pattern’ of light-staining filaments 70–80 Å in diameter embedded in a dark-staining osmiophilic amorphous matrix1. The filaments are synthesised in the inner living cell layers and are variously termed tonofilaments,α-keratin or, more accurately, keratin filaments. The subunits of the filaments of both the living cells and stratum corneum have been extracted with dissociating reagents from bovine2–4, rat5–6, mouse7, and human8epidermis. In appropriate conditions, these subunits will polymerisein vitrointo native-type keratin filaments that are uniformally70–80Å in diameter7,9. In contrast, the amorphous matrix has not been positively identified at the biochemical level, although it was recently suggested that a stratum corneum basic protein (SCBP) rich in histidine has properties consistent with a possible function as an interfilamentous matrix substance10. We show here that highly ordered structures reminiscent of the keratin pattern in the intact stratum corneum form when the SCBP and epidermal keratin filaments are combinedin vitro.