Antioxidant activity of inflorescences, leaves and fruits of three Sorbus species in relation to their polyphenolic composition

Antioxidant activity of inflorescences, leaves and fruits of three Sorbus species in relation to their polyphenolic composition
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DOI:
10.1080/14786410802636177
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发表时间:
2009-01-01
影响因子:
2.2
通讯作者:
Michel, Piotr
Michel, Piotr
中科院分区:
化学3区
文献类型:
--
作者:
Olszewska, Monika A.;Michel, Piotr

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使用三个体外测试系统(DPPH:2,2-二苯基-1-三硝基苯肼;TEAC:曲乐当量抗氧化能力和 FRAP:铁还原/抗氧化能力)评估了花果、S. aria 和 S. intermedia 的花序、叶子和果实的 70% 甲醇提取物的抗氧化潜力。发现的高活性以每克干重的微摩尔维生素E当量表示,并且对于DPPH测试,其范围为86.9-956.2;对于TEAC测定,其范围为65.3-577.2;对于FRAP方法,其范围为221.1-1915.2。这些值与总酚类、总黄酮类、总原花青素和绿原酸异构体的含量之间存在显着的线性相关性(R-2在0.75-0.98范围内),表明列出的酚类化合物是测试的抗氧化能力的决定因素。由于 S. aucuparia 花序具有优异的活性和最高的酚含量(以没食子酸当量计算的总酚含量为 11.83%,绿原酸异构体为 4.35%,原花青素为 5.01%,黄酮苷元为 1.28%),因此该植物材料具有作为天然保健品来源的最大潜力。
The antioxidant potential of 70% methanolic extracts from the inflorescences, leaves and fruits of Sorbus aucuparia, S. aria and S. intermedia was evaluated using three in vitro test systems ( DPPH: 2,2-diphenyl-1-picryl hydrazyl; TEAC: trolox equivalent antioxidant capacity and FRAP: ferric reducing/antioxidant power). High activities found were expressed as micromoles trolox equivalents per gram dry weight and were in the range of 86.9-956.2 for the DPPH test, 65.3-577.2 for the TEAC assay and 221.1-1915.2 for the FRAP method. Significant linear correlations (R-2 in the range of 0.75-0.98) between these values and the contents of total phenolics, total flavonoids, total proanthocyanidins and chlorogenic acid isomers showed that the listed phenolic compounds are determinants of the antioxidant capacity tested. As the superior activity and the highest phenolic levels (11.83% of total phenolics calculated as gallic acid equivalents, 4.35% of chlorogenic acid isomers, 5.01% of proanthocyanidins and 1.28% of flavonoid aglycones) were found for S. aucuparia inflorescence, this plant material has the greatest potential as a source for natural health products.