Apoptosis and the pattern of DNase I expression following massive small bowel resection.
Apoptosis and the pattern of DNase I expression following massive small bowel resection.
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大面积小肠切除术后细胞凋亡和 DNase I 表达模式。
DOI：
10.1006/jsre.1999.5649
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Warner,BW
中科院分区：
文献类型：
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作者：
FalconeJr,RA;Stern,LE;Kemp,CJ;Shin,CE;Erwin,CR;Warner,BW
简介大规模小肠切除术 (SBR) 后，在多种动物模型中已证实肠上皮细胞凋亡增加的组织学证据。脱氧核糖核酸酶 I (DNase I) 是细胞凋亡过程中 DNA 核内切割所必需的；因此，我们假设该基因的表达在 SBR 后会增加。方法雄性 ICR 小鼠接受 50% 近端 SBR 或假手术（肠横切/再吻合术）。 12小时以及1、3和7天后，记录肠上皮细胞增殖和凋亡率以及DNase I mRNA表达和活性。结果通过在每个时间点增殖的增加证实了SBR后的适应。肠上皮细胞增殖增加 12 小时，细胞凋亡增加 24 小时，并在第 7 天保持较高水平。与假手术小鼠相比，SBR 导致术后 24 小时 DNase I 表达和活性增加两倍，并在术后第 3 天恢复到基线。结论 大量 SBR 后早期 DNase I 表达和活性增加，但尽管肠上皮细胞凋亡和增殖率持续增加，但仍恢复到基线。这种酶可能在大量 SBR 后早期诱导细胞凋亡中发挥重要作用，但一旦在肠上皮细胞产生率和肠上皮细胞损失率之间的平衡中建立了新的设定点，这种酶就不再重要。
IntroductionFollowing massive small bowel resection (SBR), histologic evidence of increased enterocyte apoptosis has been demonstrated in several animal models. Deoxyribonuclease I (DNase I) is requisite for intranuclear cleavage of DNA during apoptosis; we therefore hypothesized that the expression of this gene would be increased following SBR.MethodsMale ICR mice underwent either 50% proximal SBR or sham surgery (bowel transection/reanastomosis). After 12 h and 1, 3, and 7 days, rates of enterocyte proliferation and apoptosis were recorded as well as DNase I mRNA expression and activity.ResultsAdaptation after SBR was confirmed at each time point by increased proliferation. Enterocyte proliferation was increased by 12 h and apoptosis was increased by 24 h and remained elevated through Day 7. When compared with sham-operated mice, SBR resulted in a twofold increase in both DNase I expression and activity at 24 h postoperatively, which returned to baseline by Postoperative Day 3.ConclusionsDNase I expression and activity are increased early following massive SBR but return to baseline despite persistent increased rates of enterocyte apoptosis and proliferation. This enzyme may be important in the early induction of apoptosis following massive SBR, but not once a new set point has been established in the balance between the rate of enterocyte production and enterocyte loss.