The use of hydrogen gas clearance for blood flow measurements in single endogenous and transplanted pancreatic islets
The use of hydrogen gas clearance for blood flow measurements in single endogenous and transplanted pancreatic islets
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DOI：
10.1016/j.mvr.2014.10.002
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发表时间：
2015-01-01
影响因子：
3.1
通讯作者：
Palm, Fredrik
中科院分区：
文献类型：
--
作者：
Barbu, Andreea;Jansson, Leif;Palm, Fredrik
胰岛的血液灌注独立于外分泌胰腺的血液灌注来调节，并且对于正常胰岛功能的多个方面是重要的，并且可能在葡萄糖耐量受损期间也是重要的。由于技术限制，单个胰岛血流难以评估。因此，我们采用了氢气冲洗技术，使用微电极，以允许这样的测量。铂微电极监测从雄性刘易斯大鼠胰腺中的单个内源性和移植的胰岛以及在雄性无胸腺小鼠的肾包膜下植入的人类和小鼠胰岛中的氢气清除。在大鼠内源性胰岛以及胰腺内移植胰岛中，血管传导性和血流值显示出高度不均匀的分布，在同一胰腺内变化6-10倍。移植于无胸腺小鼠的人和小鼠胰岛移植物的血流量比周围肾实质的血流量低约30%。本技术提供了独特的机会来研究移植后观察到的胰岛血管功能障碍，而且还允许在体内单个胰岛水平上研究遗传和环境扰动对胰岛血流的影响。(C)2014作者爱思唯尔公司出版
The blood perfusion of pancreatic islets is regulated independently from that of the exocrine pancreas, and is of importance for multiple aspects of normal islet function, and probably also during impaired glucose tolerance. Single islet blood flow has been difficult to evaluate due to technical limitations. We therefore adapted a hydrogen gas washout technique using microelectrodes to allow such measurements. Platinum micro-electrodes monitored hydrogen gas clearance from individual endogenous and transplanted islets in the pancreas of male Lewis rats and in human and mouse islets implanted under the renal capsule of male athymic mice. Both in the rat endogenous pancreatic islets as well as in the intra-pancreatically transplanted islets, the vascular conductance and blood flow values displayed a highly heterogeneous distribution, varying by factors 6-10 within the same pancreas. The blood flow of human and mouse islet grafts transplanted in athymic mice was approximately 30% lower than that in the surrounding renal parenchyma. The present technique provides unique opportunities to study the islet vascular dysfunction seen after transplantation, but also allows for investigating the effects of genetic and environmental perturbations on islet blood flow at the single islet level in vivo. (C) 2014 The Authors. Published by Elsevier Inc.