Probiotic and Safety Properties Screening ofEnterococcus faecalisfrom Healthy Chinese Infants
Probiotic and Safety Properties Screening ofEnterococcus faecalisfrom Healthy Chinese Infants
复制标题
中国健康婴儿粪肠球菌的益生菌及安全特性筛选
DOI：
10.1007/s12602-019-09625-7
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发表时间：
2020-09-01
影响因子：
4.9
通讯作者：
Han, Bei
中科院分区：
文献类型：
--
作者：
Wang, Juan;Da, Rong;Han, Bei
The aim of this study was to evaluate the probiotic characteristics and safety of sevenEnterococcus faecalisisolates from fecal samples of healthy Chinese infants. We evaluated the isolates' tolerance to low pH, survival in bile salts and NaCl, adhesion ability, biofilm formation, antimicrobial activity, toxin gene distribution, hemolysis, gelatinase activity, antibiotic resistance, and virulence toGalleria mellonella. All strains survived at pH 5.0, in 7.0% NaCl, and in 3% bile salt. Adhesion to Caco-2 cells was above 10%. Strain A3-1 had higher adhesion ability toward mucin, collagen, and BSA in vitro, better antibacterial activity, and the strongest biofilm production. We detected seven virulence genes with a distribution ofasa1(100%),cylA(71.4%),esp(85.7%),hyl(14.3%),gelE(85.7%),ace(42.9%), andagg(71.4%). Although all strains were gamma-hemolytic, none showed gelatinase activity based on physiological activity detection. All isolates were susceptible to benzylpenicillin, ampicillin, ciprofloxacin, levofloxacin, moxifloxacin, tigecycline, nitrofurantoin, linezolid, and vancomycin; they were not susceptible to erythromycin, quinupristin/dalofopine, and clindamycin. The virulence test ofG. mellonellashowed that, except for strains 106-1 and 113-1, the other strains had toxicity lower than 10%. Strain A3-1 may have the greatest potential to be developed as a probiotic.
