Endogenous protease-dependent replication of human influenza viruses in two MDCK cell lines
Endogenous protease-dependent replication of human influenza viruses in two MDCK cell lines
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DOI：
10.1007/s007050050428
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发表时间：
1998-01-01
影响因子：
2.7
通讯作者：
Yoshida, T
中科院分区：
文献类型：
--
作者：
Noma, K;Kiyotani, K;Yoshida, T
在两种MDCK细胞系MDCK（-）和MDCK（+）中研究了甲型流感病毒和乙型流感病毒的多周期复制和斑块形成以及内源性蛋白酶对其血凝素（HA）的裂解激活。在MDCK（+）细胞中测试的所有甲型流感病毒的多周期复制和斑块形成不需要外源性胰蛋白酶，而MDCK（-）细胞中的病毒则完全依赖胰蛋白酶。另一方面，在两种细胞系中，乙型流感病毒在缺乏胰蛋白酶的情况下生长良好。流感病毒的多次复制和斑块形成能力与HA前体（HA(0)）裂解为HA(1)和HA(2)相关，这表明两种细胞系都表达HA活化内源性蛋白酶(s)；MDCK（+）细胞激活流感A和流感B病毒的HA，而MDCK（-）细胞仅激活流感B病毒的HA。此外，MDCK（+）细胞的蛋白酶强烈提示存在于细胞表面和丝氨酸蛋白酶。MDCK（+）细胞可用于从临床标本中分离流感病毒和筛选抗流感病毒药物的蛋白酶抑制剂。
Multi-cycle replication and plaque formation of influenza A and B viruses and cleavage activation of their hemagglutinin (HA) by an endogenous protease(s) were examined in two MDCK cell lines, MDCK(-) and MDCK(+). No exogenous trypsin was required for multi-cycle replication and plaque formation of all the influenza A viruses tested in the MDCK(+) cell, while those of the viruses in the MDCK(-) cell were completely trypsin-dependent. In both cell lines, on the other hand, influenza B viruses grew well in the absence of trypsin. The capability of multiple replication and plaque formation of the influenza viruses correlated with cleavage of the HA precursor (HA(0)) to HA(1) and HA(2), indicating that both cell lines express an HA activating endoprotease(s); that of the MDCK(+) cell activates the HA of influenza A and B viruses, and that of the MDCK(-) cell does only the HA of influenza B virus. Furthermore, the protease of the MDCK(+) cell was strongly suggested to be present on the cell surface and a serine protease. The MDCK(+) cell would be useful fur isolation of influenza viruses from clinical specimens and for screening of protease inhibitors for anti-influenza virus drugs.