ANTIBACTERIAL ACTIVITY OF MDPB POLYMER INCORPORATED IN DENTAL RESIN
ANTIBACTERIAL ACTIVITY OF MDPB POLYMER INCORPORATED IN DENTAL RESIN
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DOI：
10.1016/0300-5712(95)93576-n
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发表时间：
1995-06-01
影响因子：
4.4
通讯作者：
MCCABE, JF
中科院分区：
文献类型：
--
作者：
IMAZATO, S;RUSSELL, RRB;MCCABE, JF
目的：我们曾报道含有新型单体甲基丙烯酰氧基十二烷基吡啶(MDPB)的牙科复合材料固化后不释放抗菌成分，但其表面仍具有抗变形链球菌的抗菌活性。为了阐明固定化MDPB的抗菌作用机制，本研究考察了MDPB聚合物的水溶性和不溶性杀菌活性，并评价了MDPB聚合物对细菌附着的影响。方法：制备MDPB均聚物溶液和MDPB与丙烯酰胺共聚物溶液，每种聚合物溶液孵育后测定7种主要口腔链球菌的存活率。为了评估不溶性MDPB聚合物的杀菌活性，将细菌与固化的未填充的含或不含MDPB的Bis-GMA基树脂盘接触，并测量活细胞回收率。结果：MDPB的水溶性均聚体及其与丙烯酰胺的共聚物对口腔链球菌均具有杀菌活性。然而，加入MDPB的固化树脂以水不溶的形式存在，几乎没有杀菌活性。包括牙菌斑形成早期定植菌种在内的链球菌在含有MDPB的固化树脂上的附着显著低于不含MDPB的对照。结论：MDPB聚合物固定化后杀菌活性降低，但树脂基材料表面的MDPB对口腔链球菌仍具有抑菌和抗黏附作用。
Objectives: Previously, we have reported that dental composite incorporating the new monomer methacryloyloxydodecylpyridinium bromide (MDPB) showed no release of antibacterial components after being cured but still exhibited antibacterial activity against Streptococcus mutans on its surface. In this study, in order to elucidate the mechanism of the antibacterial effect of immobilized MDPB, the bactericidal activity of MDPB polymer in water-soluble and -insoluble form was investigated, and the effect of MDPB polymer on bacterial attachment was estimated.Methods: Solutions of homo-polymer of MDPB and co-polymer of MDPB with acrylamide were prepared and the viability of seven major oral streptococci was determined after incubation with each polymer solution. For the estimation of bactericidal activity of insolubilized MDPB polymer, bacteria were kept in contact with cured unfilled Bis-GMA-based resin discs with or without MDPB, and the recovery of viable cells was measured. Attachment of streptococci to cured resin discs with or without MDPB was also compared using radiolabelled bacteria.Results: Water-soluble homo-polymer of MDPB and co-polymer with acrylamide showed bactericidal activity against oral streptococci. However, cured resin incorporating MDPB, which is in water-insoluble form, had little bactericidal activity. Attachment of streptococci, including species which are early colonizers in dental plaque formation, to the cured resin containing MDPB was significantly less than to the control without MDPB.Conclusions: These results indicate that the bactericidal activity of MDPB polymer is reduced after immobilization, but MDPB on the surface of a resin-based material still shows a bacteriostatic effect and antiadhesion property against oral streptococci.