Adaptive optics enables 3D STED microscopy in aberrating specimens
Adaptive optics enables 3D STED microscopy in aberrating specimens
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DOI：
10.1364/oe.20.020998
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发表时间：
2012-09-10
期刊：
影响因子：
3.8
通讯作者：
Booth, Martin J.
中科院分区：
文献类型：
--
作者：
Gould, Travis J.;Burke, Daniel;Booth, Martin J.
受激射电子耗尽(STED)显微镜可以实现具有无限衍射分辨率的荧光远场成像。不幸的是，将这项技术扩展到厚样品的三维(3D)成像已经被样品诱导的像差所阻碍。在这里，我们介绍了自适应光学在STED显微镜中的首次实现，以允许在强像差成像条件下进行3D超分辨率成像，例如由厚生物组织引入的那些成像条件。(C)2012年美国光学学会
Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implementation of adaptive optics in STED microscopy to allow 3D super-resolution imaging in strongly aberrated imaging conditions, such as those introduced by thick biological tissue. (C) 2012 Optical Society of America