Volatile anesthetics block actin-based motility in dendritic spines
Volatile anesthetics block actin-based motility in dendritic spines
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DOI：
10.1073/pnas.96.18.10433
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发表时间：
1999-08-31
影响因子：
11.1
通讯作者：
Matus, A
中科院分区：
文献类型：
--
作者：
Kaech, S;Brinkhaus, H;Matus, A
树突棘是大脑中大多数兴奋性突触的突触后接触部位。根据动物的经验或行为状态，脊椎的形状各异。最近我们展示了活体神经元上的棘可以在几秒钟内改变形状，这一过程取决于肌动蛋白聚合。我们现在已经发现，这种形态的可塑性被吸入麻醉剂在临床上有效的浓度所阻断。这些挥发性化合物还阻断成纤维细胞中肌动蛋白为基础的运动，表明它们的作用独立于神经元特异性成分，从而确定肌动蛋白细胞骨架是麻醉作用的一般细胞靶点。这些观察结果表明，在全身麻醉期间，脑突触的肌动蛋白动力学受到抑制，吸入麻醉剂能够影响脑兴奋性突触的形态可塑性。
Dendritic spines form the postsynaptic contact sites for most excitatory synapses in the brain. Spines occur in a wide range of different shapes that can vary depending on an animal's experience or behavioral status. Recently we showed that spines on living neurons can change shape within seconds in a process that depends on actin polymerization. We have now found that this morphological plasticity is blocked by inhalational anesthetics at concentrations at which they are clinically effective. These volatile compounds also block actin-based motility in fibroblasts, indicating that their action is independent of neuron-specific components and thus identifying the actin cytoskeleton as a general cellular target of anesthetic action. These observations imply that inhibition of actin dynamics at brain synapses occurs during general anesthesia and that inhalational anesthetics are capable of influencing the morphological plasticity of excitatory synapses in the brain.